Determining the polysaccharide composition of plant cell walls
Determining the polysaccharide composition of plant cell walls
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DOI：
10.1038/nprot.2012.081
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发表时间：
2012-09-01
期刊：
影响因子：
14.8
通讯作者：
Bacic, Antony
中科院分区：
文献类型：
--
作者：
Pettolino, Filomena A.;Walsh, Cherie;Bacic, Antony
植物细胞壁是主要由多糖组成的化学复杂结构。对这些细胞壁多糖的详细分析对于我们理解植物发育和我们在食品，农业，织物，木材，生物燃料和生物复合材料行业中使用植物生物质（主要是壁材料）至关重要。我们目前的分析技术，不仅要确定个别细胞壁多糖的精细化学结构，而且要估计细胞壁制剂的整体多糖组成。该程序涵盖了细胞壁的制备，连同气相色谱-质谱（GC-MS）为基础的方法，既为单糖作为其挥发性的糖醇乙酸酯衍生物的分析和甲基化分析，以确定单糖残基之间的连接位置作为其挥发性的部分甲基化的糖醇乙酸酯衍生物。分析时间将根据所使用的方法和组织类型而变化，范围从简单中性糖组成的2天到羧基还原/甲基化连接分析的2周。
The plant cell wall is a chemically complex structure composed mostly of polysaccharides. Detailed analyses of these cell wall polysaccharides are essential for our understanding of plant development and for our use of plant biomass (largely wall material) in the food, agriculture, fabric, timber, biofuel and biocomposite industries. We present analytical techniques not only to define the fine chemical structures of individual cell wall polysaccharides but also to estimate the overall polysaccharide composition of cell wall preparations. The procedure covers the preparation of cell walls, together with gas chromatography-mass spectrometry (GC-MS)-based methods, for both the analysis of monosaccharides as their volatile alditol acetate derivatives and for methylation analysis to determine linkage positions between monosaccharide residues as their volatile partially methylated alditol acetate derivatives. Analysis time will vary depending on both the method used and the tissue type, and ranges from 2 d for a simple neutral sugar composition to 2 weeks for a carboxyl reduction/methylation linkage analysis.