Microporous methacrylated glycol chitosan-montmorillonite nanocomposite hydrogel for bone tissue engineering
Microporous methacrylated glycol chitosan-montmorillonite nanocomposite hydrogel for bone tissue engineering
复制标题
用于骨组织工程的微孔甲基丙烯酸乙二醇壳聚糖-蒙脱石纳米复合水凝胶
DOI：
10.1038/s41467-019-11511-3
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发表时间：
2019-08-06
影响因子：
16.6
通讯作者：
Lee, Min
中科院分区：
文献类型：
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作者：
Cui, Zhong-Kai;Kim, Soyon;Lee, Min
注射水凝胶可以填补不规则缺损，促进原位组织再生。在三维微环境中引导干细胞分化用于骨再生的能力仍然是一个挑战。在本研究中，我们通过引入二维纳米粘土颗粒和嵌入化学，成功地在原位形成水凝胶中构建了一种相互连接的微孔网络光交联壳聚糖。纳米硅酸盐的存在增加了杨氏模量，减慢了水凝胶的降解速度。结果表明，强化水凝胶可促进包被间充质干细胞的体外增殖、附着和分化。此外，我们在小鼠颅骨缺损模型中探讨了纳米工程水凝胶在体内的作用。我们的研究结果证实，壳聚糖-蒙脱土水凝胶能够招募天然细胞并促进颅骨愈合，而无需额外的治疗剂或干细胞，这表明它们具有组织工程潜力。
Injectable hydrogels can fill irregular defects and promote in situ tissue regrowth and regeneration. The ability of directing stem cell differentiation in a three-dimensional microenvironment for bone regeneration remains a challenge. In this study, we successfully nanoengineer an interconnected microporous networked photocrosslinkable chitosan in situforming hydrogel by introducing two-dimensional nanoclay particles with intercalation chemistry. The presence of the nanosilicates increases the Young's modulus and stalls the degradation rate of the resulting hydrogels. We demonstrate that the reinforced hydrogels promote the proliferation as well as the attachment and induced the differentiation of encapsulated mesenchymal stem cells in vitro. Furthermore, we explore the effects of nanoengineered hydrogels in vivo with the critical-sized mouse calvarial defect model. Our results confirm that chitosan-montmorillonite hydrogels are able to recruit native cells and promote calvarial healing without delivery of additional therapeutic agents or stem cells, indicating their tissue engineering potential.