Organ distribution of quantum dots after intraperitoneal administration, with special reference to area-specific distribution in the brain
Organ distribution of quantum dots after intraperitoneal administration, with special reference to area-specific distribution in the brain
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DOI：
10.1088/0957-4484/21/33/335103
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发表时间：
2010-08-20
期刊：
影响因子：
3.5
通讯作者：
Fushiki, Shinji
中科院分区：
文献类型：
--
作者：
Kato, Shingo;Itoh, Kyoko;Fushiki, Shinji
量子点因其在生物传感、离体活细胞成像和活体动物靶向等方面的潜在应用而闻名。大脑是一个具有挑战性的器官，因为血脑屏障（BBB）的功能是保护身体免受外源性物质的侵害。本研究采用ICP-MS和共聚焦显微镜结合光谱分析的方法，对雄性ICR小鼠腹腔注射卡托普利（captopril-conjugated QDs, QDs-cap）作为确定QDs组织定位的模型系统进行了生物偶联QDs分布的评估。我们已经证明，注射后6小时，腹腔注射QDs-cap通过体循环进入肝、脾、肾和脑。QDs-cap主要分布于肝、肾和脑的血管内，但也有少量分布于实质，尤其是在脑内。在临床应用于人类之前，需要仔细研究量子点在大脑中的急性和慢性毒性。
Quantum dots (QDs) are well known for their potential application in biosensing, ex vivo live-cell imaging and in vivo animal targeting. The brain is a challenging organ for drug delivery, because the blood brain barrier (BBB) functions as a gatekeeper guarding the body from exogenous substances. Here, we evaluated the distribution of bioconjugated QDs, i.e., captopril-conjugated QDs (QDs-cap) following intraperitoneal injection into male ICR mice as a model system for determining the tissue localization of QDs, employing ICP-MS and confocal microscopy coupled with spectrometric analysis. We have demonstrated that intraperitoneally administered QDs-cap were delivered via systemic blood circulation into liver, spleen, kidney and brain at 6 h after injection. QDs-cap were located predominantly inside the blood vessels in the liver, kidney and brain, but a few were distributed in the parenchyma, especially noteworthy in the brain. Careful studies on acute as well as chronic toxicity of QDs in the brain are required prior to clinical application to humans.