Properties of different lactic acid bacteria isolated from Apis mellifera L. bee-gut
Properties of different lactic acid bacteria isolated from Apis mellifera L. bee-gut
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DOI：
10.1016/j.micres.2010.01.003
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发表时间：
2011-01-01
影响因子：
6.7
通讯作者：
Apella, Maria C.
中科院分区：
文献类型：
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作者：
Carina Audisio, M.;Torres, Maria J.;Apella, Maria C.
属于乳杆菌属的8个菌株。肠球菌属5株。从工蜂蜜蜂的肠道中分离到2株。16S rRNA序列分析表明，AJ5、IG9、A15和CRL1647菌株与约翰逊乳杆菌的同源性为99%，SM21与屎肠球菌的同源性为99%。强生乳杆菌CRL1647、AJ5和IG9产酸量较高(在177~275 mm之间)，在体外对不同的人类食源性致病菌和美国污浊因子拟青霉幼虫均有抑制作用。该细菌对44 mM的这种代谢物抑制乳酸的影响最为敏感。强生乳杆菌CRL1647、AJ5和IG9也表现出重要的表面性质。这些细胞表现出77%到93%的自动聚集。对凝集因子的化学性质的初步研究表明，每个约翰逊乳杆菌菌株表面涉及的分子都相当复杂，主要涉及某种多肽性质。粪肠球菌SM21产生具有抗李斯特氏菌作用的细菌素类化合物。通过SDS-PAGE分析，在6.0-7.5KDA附近检测到一条带，并通过聚合酶链式反应扩增出EntA、B和P结构基因。首次分离、鉴定、培养了与蜜蜂肠道相关的约翰逊乳杆菌和屎乳杆菌，并报道了它们的一些功能特性。(C)2010年爱思唯尔股份有限公司。版权所有。
Eight strains belonging to Lactobacillus spp. and five to Enterococcus spp. were isolated from the gut of worker Apis mellifera L. bees. Studies based on 16S rRNA sequencing revealed that AJ5, IG9, A15 and CRL1647 strains had a 99% identity with Lactobacillus johnsonii, while SM21 showed a 99% similarity with Enterococcus faecium. L. johnsonii CRL1647, AJ5 and IG9 were high lactic acid producers (values were between 177 and 275 mM), and in vitro they inhibited different human food-borne pathogens and Paenibacillus larvae, the American foulbrood agent. This bacterium was the most sensitive to the lactic acid effect being inhibited by 44 mM of this metabolite. L. johnsonii CRL1647, AJ5 and IG9 also presented important surface properties. These cells showed between 77% and 93% of auto-aggregation. The preliminary study of the chemical nature of the aggregating factors revealed that the molecules involved in the surface of each L. johnsonii strain were quite complex; and something of a peptidic nature was mainly involved. E. faecium SM21 produced bacteriocin-like compounds with anti-Listeria effects. Furthermore, a band close to 6.0-7.5 kDA was detected by SDS-PAGE studies, and the entA, B and P structural genes were amplified by PCR reactions. For the first time, bee-gut associated L. johnsonii and E. faecium strains have been isolated, identified, cultivated and some of their functional properties reported. (C) 2010 Elsevier GmbH. All rights reserved.