DAF1, A MUTANT-GENE AFFECTING SIZE CONTROL, PHEROMONE ARREST, AND CELL-CYCLE KINETICS OF SACCHAROMYCES-CEREVISIAE
DAF1, A MUTANT-GENE AFFECTING SIZE CONTROL, PHEROMONE ARREST, AND CELL-CYCLE KINETICS OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/mcb.8.11.4675
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发表时间：
1988-11-01
影响因子：
5.3
通讯作者：
CROSS, FR
中科院分区：
文献类型：
--
作者：
CROSS, FR
交配信息素α-该因子将酿酒酵母MATa细胞阻滞在细胞周期的G1期。在G1中也施加了尺寸控制，因为细胞在达到临界尺寸之前不会退出G1。显性突变（DAF 1 -1）引起α-β-CD和α-CD两者。因子抗性和小细胞大小（体积约为野生型的0.6倍）的突变体已经被分离并通过遗传和分子克隆进行表征。几个α-因子诱导的mRNA在daf 1+和DAF 1 -1细胞中诱导相等。DAF 1 -1突变由daf 1+编码序列三分之二处的终止密码子组成。染色体缺失的DAF 1基因易位产生的细胞体积增加约1.5倍，因此，DAF 1 -1可能是一个过度活跃或失调的等位基因的非必需基因参与G1的大小控制。DAF 1 -1的多个拷贝也大大减少了细胞周期的G1期的持续时间。
The mating pheromone .alpha.-factor arrests Saccharomyces cerevisiae MATa cells in the G1 phase of the cell cycle. Size control is also exerted in G1, since cells do not exit G1 until they have attained a critical size. A dominant mutation (DAF1-1) which causes both .alpha.-factor resistance and small cell size (volume about 0.6-fold that of the wild type) has been isolated and characterized genetically and by molecular cloning. Several .alpha.-factor-induced mRNAs were induced equivalently in daf1+ and DAF1-1 cells. The DAF1-1 mutation consisted of a termination codon two-thirds of the way through the daf1+ coding sequence. A chromosomal deletion of DAF1 produced by gene transplacement increased cell volume about 1.5-fold; thus, DAF1-1 may be a hyperactive or deregulated allele of a nonessential gene involved in G1 size control. Multiple copies of DAF1-1 also greatly reduced the duration of the G1 phase of the cell cycle.