The 1.45 Å resolution structure of the cryptogein-cholesterol complex:: a close-up view of a sterol carrier protein (SCP) active site
The 1.45 Å resolution structure of the cryptogein-cholesterol complex:: a close-up view of a sterol carrier protein (SCP) active site
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DOI：
10.1107/s0907444902011745
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发表时间：
2002-09-01
影响因子：
2.2
通讯作者：
Prangé, T
中科院分区：
文献类型：
--
作者：
Lascombe, MB;Ponchet, M;Prangé, T
隐地蛋白是由植物寄生卵菌隐地疫霉（Phytophthora cryptogea）产生的小的10 kDa激发子。该蛋白质还显示出固醇载体活性。天然蛋白质以空间群P4（1）22结晶，晶胞参数a = B = 46.51，c = 134.9埃（衍射极限：2.1埃）。其与胆固醇的复合物的晶体属C222（1）空间群，晶胞参数a = 30.96，B = 94.8，c = 65.3埃，分辨率提高到1.45埃。大的内部非特异性疏水腔能够容纳多种3-β-羟基甾醇。隐地蛋白可能作为固醇穿梭帮助病原体生长并完成其生命周期。
Cryptogein is a small 10 kDa elicitor produced by the phytoparasitic oomycete Phytophthora cryptogea. The protein also displays a sterol carrier activity. The native protein crystallizes in space group P4(1)22, with unit-cell parameters a = b = 46.51, c = 134.9 Angstrom (diffraction limit: 2.1 Angstrom). Its complex with cholesterol crystallizes in space group C222(1), with unit-cell parameters a = 30.96, b = 94.8, c = 65.3 Angstrom and a resolution enhanced to 1.45 Angstrom. The large inner non-specific hydrophobic cavity is able to accommodate a large variety of 3-beta-hydroxy sterols. Cryptogein probably acts as a sterol shuttle helping the pathogen to grow and complete its life cycle.