The sintered microsphere matrix for bone tissue engineering:: In vitro osteoconductivity studies
The sintered microsphere matrix for bone tissue engineering:: In vitro osteoconductivity studies
复制标题
DOI：
10.1002/jbm.10201
复制
发表时间：
2002-09-05
期刊：
影响因子：
--
通讯作者：
Laurencin, CT
中科院分区：
文献类型：
--
作者：
Borden, M;Attawia, M;Laurencin, CT
组织工程方法已被用于设计用于骨修复的三维合成基质。在体外环境中评价了一种新型聚合物骨移植替代物的骨传导性和降解特性。以聚乳酸-羟基乙酸共聚物[PLAGA]为原料，采用微球烧结技术制备三维多孔骨再生支架。将成骨细胞和成纤维细胞接种到50：50的PLAGA支架上。通过扫描电子显微镜进行的形态学评价表明，两种细胞类型附着并在支架上扩散。细胞迁移通过基质使用细胞质延伸桥接结构。横截面图像表明，细胞增殖已渗透到基质中约700 μ m的表面。细胞/基质构建体表面的检查表明，细胞增殖在细胞培养14天时已经包围了基质的孔。以优化聚合物组成和聚合物分子量为目的，利用基质进行降解研究。结果表明，降解的烧结基质是依赖于分子量，共聚物的比例，和孔体积。根据该数据，确定初始分子量为100，000的75：25 PLAGA具有最佳降解曲线。这些研究表明，烧结的微球基质具有骨传导结构，能够充当具有适合骨再生的降解特征的细胞支架。(C)2002 Wiley Periodicals，Inc.
A tissue engineering approach has been used to design three-dimensional synthetic matrices for bone repair. The osteoconductivity and degradation profile of a novel polymeric bone-graft substitute was evaluated in an in vitro setting. Using the copolymer poly(lactide-co-glycolide) [PLAGA], a sintering technique based on microsphere technology was used to fabricate three-dimensional porous scaffolds for bone regeneration. Osteoblasts and fibroblasts were seeded onto a 50:50 PLAGA scaffold. Morphologic evaluation through scanning electron microscopy demonstrated that both cell types attached and spread over the scaffold. Cells migrated through the matrix using cytoplasmic extensions to bridge the structure. Cross-sectional images indicated that cellular proliferation had penetrated into the matrix approximately 700 mum from the surface. Examination of the surfaces of cell/matrix constructs demonstrated that cellular proliferation had encompassed the pores of the matrix by 14 days of cell culture. With the aim of optimizing polymer composition and polymer molecular weight, a degradation study was conducted utilizing the matrix. The results demonstrate that degradation of the sintered matrix is dependent on molecular weight, copolymer ratio, and pore volume. From this data, it was determined that 75:25 PLAGA with an initial molecular weight of 100,000 has an optimal degradation profile. These studies show that the sintered microsphere matrix has an osteoconductive structure capable of functioning as a cellular scaffold with a degradation profile suitable for bone regeneration. (C) 2002 Wiley Periodicals, Inc.