The relationship between bifidobacteria and allergic asthma and/or allergic dermatitis: A prospective study of 0-3 years-old children in Turkey
The relationship between bifidobacteria and allergic asthma and/or allergic dermatitis: A prospective study of 0-3 years-old children in Turkey
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DOI：
10.1016/j.anaerobe.2014.05.006
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发表时间：
2014-08-01
期刊：
影响因子：
2.3
通讯作者：
Kocazeybek, Bekir S.
中科院分区：
文献类型：
--
作者：
Akay, Hatice Kubra;Tokman, Hrisi Bahar;Kocazeybek, Bekir S.
双歧杆菌是对人类有益的细菌。这些细菌在预防传染病和调节免疫反应方面特别有效。结果表明，在新生儿中，定居双歧杆菌属物种的粪便分布影响过敏性疾病的患病率。本研究旨在比较过敏儿童和健康儿童粪便中双歧杆菌的种类，以检测粪便分布的种类水平差异。粪便样本来自99名0至3岁的儿童，其临床症状和实验室报告与特应性皮炎和过敏性哮喘一致。样本还从102名健康儿童中获得，这些儿童在年龄和性别方面与病例组相似。采用培养法分离双歧杆菌，并采用API 20 A.此外，7个独特的种特异性引物被用于双链菌的分子特征。McNemar检验用于统计分析，并且p < 0.05被认为是显著的。过敏儿童中检出长双歧杆菌11例（11.1%），健康儿童中检出长双歧杆菌31例（30.3%），两组B菌检出率差异有统计学意义。两组之间的X-2：11.2，p < 0.001。然而，在健康和过敏儿童的粪便样本中，其他双歧杆菌的患病率没有显着差异。（p > 0.05）。分离出的B。longum的研究表明，该物种通过预防哮喘和过敏性皮炎的发展而有利于宿主。基于这些结果，我们建议按照国家特定的双歧杆菌物种密度生产益生菌将改善公众健康。因此，需要进行收集广泛数据集的特定国家前瞻性病例对照研究。(C)2014爱思唯尔有限公司版权所有。
Bifidobacteria are beneficial bacteria for humans. These bacteria are particularly effective at protecting against infectious diseases and modulating the immune response. It was shown that in newborns, the fecal distribution of the colonizing Bifidobacterium species influences the prevalence of allergic diseases. This study aimed to compare the faecal Bifidobacterium species of allergic children to those of healthy children to detect species level differences in faecal distribution. Stool samples were obtained from 99 children between 0 and 3 years of age whose clinical symptoms and laboratory reports were compatible with atopic dermatitis and allergic asthma. Samples were also obtained from 102 healthy children who were similar to the case group with respect to age and sex. Bifidobacteria were isolated by culture and identified at the genus level by API 20 A. In addition, 7 unique species-specific primers were used for the molecular characterization of bifidobacteria. The McNemar test was used for statistical analyses, and p < 0.05 was accepted as significant. Bifidobacterium longum was detected in 11(11.1%) of the allergic children and in 31 (30.3%) of the healthy children.Statistical analysis revealed a significant difference in the prevalence of B. longum between these two groups (X-2: 11.2, p < 0.001). However, no significant differences in the prevalence of other Bifidobacterium species were found between faecal samples from healthy and allergic children. (p > 0.05). The significant difference in the isolation of B. longum from our study groups suggests that this species favors the host by preventing the development of asthma and allergic dermatitis. Based on these results, we propose that the production of probiotics in accordance with country-specific Bifidobacterium species densities would improve public health. Thus, country-specific prospective case control studies that collect broad data sets are needed. (C) 2014 Elsevier Ltd. All rights reserved.