[Morphological observation of combined-culture of tendon cell or fibroblast of rabbit with artificial materials in vitro].
[Morphological observation of combined-culture of tendon cell or fibroblast of rabbit with artificial materials in vitro].
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兔肌腱细胞或成纤维细胞与人工材料体外联合培养的形态观察
DOI：
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发表时间：
1997
影响因子：
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通讯作者：
W. Peng
中科院分区：
文献类型：
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作者：
Q. Zhang;Z. Yang;W. Peng
为探讨兔肌腱细胞或成纤维细胞与人工材料的相容性和生长情况，分别与碳纤维、涤纶、甲壳素复合培养。结果表明：在体外，碳纤维与两种细胞相容性良好，细胞粘附能力良好。在涤纶上几乎没有细胞生长。几丁质抑制细胞的生长。无论是肌腱细胞还是成纤维细胞，在碳纤维上的粘附量均远大于涤纶或甲壳素。当三种材料交织在一起时，细胞产生的胶原纤维以平行于碳纤维的方向排列。随着时间的推移，碳纤维上的细胞分布均匀，呈网络状包裹材料，这表明碳纤维是制备活体人工肌腱和韧带的良好材料。
In order to investigate the compatibility and growth between the tendon cell or fibroblast of rabbit and artificial materials, the combined-culture of the two cells with the carbon fiber, terylene and chitin was observed respectively. Results showed as following: in vitro, the compatibility of carbon fiber with these two cells was well, cell-adhesion ability was good as well. Few cells grew on terylene. Chitin inhibited the growth of either cells. No matter the tendon cell or the fibroblast, the amount of cells adhering on the carbon fiber was far more than that on terylene or chitin. When the three materials were interlaced together, the collagen fibers produced by the cells were arranged in direction parallel to the carbon fibers. As the time elapsed, the cells on the carbon fiber distributed evenly and enveloped the material in network-like fashion, this suggested that carbon fiber was a good material for producing living artificial tendon and ligament.