ECTOPIC EXPRESSION OF GAMMA-INTERFERON IN THE EYE PROTECTS TRANSGENIC MICE FROM INTRAOCULAR HERPES-SIMPLEX VIRUS TYPE-1 INFECTIONS
ECTOPIC EXPRESSION OF GAMMA-INTERFERON IN THE EYE PROTECTS TRANSGENIC MICE FROM INTRAOCULAR HERPES-SIMPLEX VIRUS TYPE-1 INFECTIONS
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DOI：
10.1128/jvi.68.9.5556-5567.1994
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发表时间：
1994-09-01
影响因子：
5.4
通讯作者：
SARVETNICK, N
中科院分区：
文献类型：
--
作者：
GEIGER, K;HOWES, EL;SARVETNICK, N
Transgenic (rho gamma) mice provide a model for studying the influence of gamma interferon (IFN-gamma) produced in the eye on ocular and cerebral viral infection. To establish this model, we injected BALI/c- and C57BL/6-derived transgenic and nontransgenic mice of different ages intravitreally with herpes simplex virus type 1 (HSV-1) strain F. Eye and brain tissues of these mice were assessed for pathological and immunocytochemical changes. HSV-1 infection induced severe retinitis of the injected eyes and infection of the brain in all mice. In transgenic mice inoculated with HSV-1, the left, nontreated eyes were protected from retinitis, whereas nontransgenic mice developed bilateral retinitis. Additional intravitreal injection of IFN-gamma with the virus protected the noninoculated eyes of nontransgenic mice. Three-week-old nontransgenic mice died from HSV-1 infection, whereas transgenic mice of the same age and nontransgenic mice intravitreally treated with IFN-gamma survived. Ocular IFN-gamma production increased the extent of inflammation in transgenic mice but did not have a significant influence on the growth of HSV-1 until day 3 after inoculation and did not influence the neuroinvasion of this virus. Thus, the effects of IFN-gamma were not caused by an early block of viral replication. Possible mechanisms of IFN-gamma action include activation of the immune response, alteration of the properties of the virus, and direct protection of neurons.
