Simultaneous determination of catecholamines and unconjugated 3,4-dihydroxyphenylacetic acid in brain tissue by ion-pairing reverse-phase high-performance liquid chromatography with electrochemical detection.
Simultaneous determination of catecholamines and unconjugated 3,4-dihydroxyphenylacetic acid in brain tissue by ion-pairing reverse-phase high-performance liquid chromatography with electrochemical detection.
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离子对反相高效液相色谱电化学检测法同时测定脑组织中的儿茶酚胺和未结合的 3,4-二羟基苯乙酸。
DOI：
10.1016/0003-2697(80)90101-3
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发表时间：
1980
影响因子：
2.9
通讯作者：
Barchas,JD
中科院分区：
文献类型：
--
作者：
Mefford,IN;Gilberg,M;Barchas,JD
建立了一种快速、灵敏的同时测定大鼠脑组织中儿茶酚胺和3,4-二羟基苯乙酸的方法。该方法很简单，只涉及组织破坏，将儿茶酚吸附到氧化铝上，解吸，并注入反相高效液相色谱系统。电化学检测具有选择性和高灵敏度。在儿茶酚胺的测定中加入3,4-二羟基苯乙酸，可以观察到药理学操作对体内单胺氧化酶活性和/或多巴胺周转的影响，以及对儿茶酚胺浓度的影响。
A rapid, sensitive method was developed for the simultaneous assay of catecholamines and 3,4-dihydroxyphenylacetic acid in rat brain tissue. The method is simple, involving only tissue disruption, adsorption of the catechols onto alumina, desorption, and injection into a reverse-phase high-performance liquid chromatography system. Selectivity and high sensitivity are obtained using electrochemical detection. The addition of 3,4-dihydroxyphenylacetic acid determination to assays for catecholamines allows one to observe effects of pharmacological maniqulations on in vivo monoamine oxidase activity and/or turnover of dopamine as well as effects on catecholamine concentrations.