Maturation of jejunoileal gradients in rat intestine: the role of intraluminal nutrients.
Maturation of jejunoileal gradients in rat intestine: the role of intraluminal nutrients.
复制标题
大鼠肠道空肠油梯度的成熟：腔内营养物的作用。
DOI：
10.1159/000243891
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Kwong,LK
中科院分区：
文献类型：
--
作者：
Castillo,RO;Feng,JJ;Stevenson,DK;Kwong,LK
空回肠功能的梯度被认为是在大鼠出生后第三周肠道成熟时建立的。为了探讨空肠回肠梯度的正常发育，以及腔内营养素的缺乏或提供形式是否影响空肠回肠梯度的发育，对正常断奶乳鼠的粘膜DNA、蛋白质、乳糖酶和蔗糖酶的梯度进行了研究，并与无腔内营养的大鼠和接受元素营养的大鼠的梯度进行了比较。在哺乳动物中，先前存在的空肠DNA和蛋白质梯度在整个断奶期间持续存在，乳糖酶梯度由回肠功能迅速下降形成，蔗糖酶梯度由空肠活动迅速增加形成。管腔内元素营养素导致空肠回肠形成与正常断奶大鼠肠道相似的梯度。腔内营养素的缺乏没有导致蔗糖酶在空肠回肠梯度表达上的质的差异，但元素营养的提供导致了该酶在空肠回肠中的差异增加。腔内营养素的缺乏导致DNA没有梯度，空肠蛋白质差异不明显，回肠乳糖酶延迟成熟下降，这阻止了空肠回肠酶梯度的发展。这些研究表明，对于所研究的参数，在成熟的大鼠肠道中形成空肠回肠梯度需要腔内营养，无论它们以何种形式提供给它们的正常表达。
Jejunoileal gradients of intestinal function are thought to be established during the third week of life in the rat when postnatal intestinal maturation occurs. In order to investigate the normal development of jejunoileal gradients and whether either the absence of intraluminal nutrients or the form in which they are provided affected the development of jejunoileal gradients, gradients for mucosal DNA, protein, lactase and sucrase were studied in suckling rats undergoing normal weaning and compared to gradients in rats receiving no intraluminal nutrients or rats receiving nutrients in elemental form. In suckling animals, preexisting jejunoileal gradients for DNA and protein persisted through the weaning period, gradients for lactase formed by rapid decline of ileal function and sucrase gradients formed by rapid increase in jejunal activities. Intraluminal nutrients in elemental form resulted in the formation of jejunoileal gradients similar to those in intestines of normally weaned rats. The lack of intraluminal nutrients resulted in no qualitative differences in the expression of jejunoileal gradients for sucrase, but provision of elemental nutrients resulted in increased jejunoileal differences for this enzyme. The lack of intraluminal nutrients resulted in no gradients for DNA, less pronounced jejunoileal differences for protein and delayed maturational decline of ileal lactase which prevented development of jejunoileal gradients for the enzyme. These studies indicate that the formation of jejunoileal gradients in the maturing rat intestine for the parameters investigated require intraluminal nutrients regardless of the form in which they are provided for their normal expression.