Protection of rat artery grafts from tissue damage by voltage-applied supercooling
Protection of rat artery grafts from tissue damage by voltage-applied supercooling
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DOI：
10.1007/s00795-021-00310-9
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发表时间：
2022-02
影响因子：
1.8
通讯作者：
A. Kuro;N. Morimoto;Tomoya Hara;Yuki Matsuoka;Michika Fukui;Masakatsu Hihara;K. Kusumoto;N. Kakudo
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文献类型：
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作者：
A. Kuro;N. Morimoto;Tomoya Hara;Yuki Matsuoka;Michika Fukui;Masakatsu Hihara;K. Kusumoto;N. Kakudo
低于 0°C 的过冷保存允许在未冷冻状态下储存可移植源。与传统4°C保存相比，这可以通过改善代谢抑制和器官保存来提高保存的安全性和有效性。我们开发了一种使用施加电压的装置而无需灌注的过冷技术。我们在大鼠动脉移植模型中检查了我们的过冷保存技术的保存效果。我们的技术在施加 1000 V 电压时产生 -2 °C 的过冷状态。发现使用所提出的方法储存的大鼠动脉组织细胞的活力高于普通条件下的活力。与普通条件下的保存相比，在−2°C 下施加电压过冷保存的股动脉血管内皮损伤减少。过冷保存后，动脉移植物成功实现移植物通畅，移植后 1 周的结果（包括血栓形成）优于传统 4 °C 保存。在 −2°C 和 1000V 下过冷动脉保存有望大大延长保存时间并改善移植后的结果。
Supercooling preservation below 0 °C allows the storage of the transplantable sources in an unfrozen state. This can improve the safety and efficacy of storage by improving the inhibition of metabolism and organ preservation in comparison with conventional preservation at 4 °C. We have developed a supercooling technique using a voltage-applied apparatus without perfusion. We examined the preservation effects of our supercooling preservation technique in a rat model of artery transplantation. Our technique produces a supercooled state at − 2 °C with application of 1000 V. The viability of tissue cells from rat arteries was found to be higher with storage using the proposed method than that under ordinary conditions. Damage to the vascular endothelium of the femoral artery preserved by voltage-applied supercooling at − 2 °C was reduced compared to storage under ordinary conditions. Artery graft revival was successfully achieved with graft patency after supercooling preservation, and 1 week outcomes for post-transplanted grafts, including thrombosis, were better with supercooling preservation than with conventional 4 °C preservation. Supercooling artery preservation at − 2 °C with 1000 V promises to greatly prolong preservation time and improve post-transplant outcomes.