Determination of biologically active substances in roasted coffees using a diode-array HPLC system
Determination of biologically active substances in roasted coffees using a diode-array HPLC system
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DOI：
10.2116/analsci.20.325
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发表时间：
2004-02-01
影响因子：
1.6
通讯作者：
Takai, N
中科院分区：
文献类型：
--
作者：
Minamisawa, M;Yoshida, S;Takai, N
采用自制的溶胶-凝胶柱和ODS-2柱，采用高效液相色谱/二极管阵列法，对几种烘焙咖啡中的生物活性物质烟酸、胡芦巴碱、咖啡因、喹啉酸、单宁酸和焦性没食子酸进行了同时定量分析。一个简单的方法，同时定量分析的生物活性物质的咖啡酿造成为可行的HPLC/二极管阵列系统与溶胶-凝胶柱在210 nm的单一波长。当两个溶胶-凝胶柱连接时，R_i值大于1.05。此外，从市售咖啡豆中提取的酿造物中烟酸的洗脱行为表明了烘焙过程中的热分解过程，并表明了全城市烘焙咖啡的最大值。
We studied the simultaneous quantitative analysis of biologically active substances, such as nicotinic acid, trigonelline, caffeine, qunolinic acid and tannic acid and pyrogallic acid, in several roasted coffees by an HPLC/diode-array system with a home-made sol-gel and ODS-2 columns. A simple method for simultaneous quantitative analysis of biologically active substances in the coffee brew became feasible by an HPLC/diode-array system with a sol-gel column at a single wavelength of 210 nm. The most efficient condition of the R, value was above 1.05 when two sol-gel columns were connected. In addition, the elution behavior of nicotinic acid in brew extracted from commercially available coffee beans suggests the thermal decomposition process during roasting, and indicated the maximum value for full city roasted coffee.