Babesia bovis:: Effects of cysteine protease inhibitors on in vitro growth
Babesia bovis:: Effects of cysteine protease inhibitors on in vitro growth
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DOI：
10.1016/j.exppara.2007.04.009
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发表时间：
2007-10-01
影响因子：
2.1
通讯作者：
Igarashi, Ikuo
中科院分区：
文献类型：
--
作者：
Okubo, Kazuhiro;Yokoyama, Naoaki;Igarashi, Ikuo
In the present study, we examined the effects of four kinds of cysteine protease inhibitors (E64, E64d, leupeptin, and ALLN) on the in vitro asexual growth of Babesia bovis. Of these, only the lipophilic inhibitors, E64d and ALLN, were found to effectively inhibit the growth of B. bovis. In further experiments, E64d, but not ALLN, significantly suppressed the parasite's invasion of host erythrocytes, while both chemicals, especially ALLN, inhibited the parasite's replication within the infected erythrocytes. These data suggested the presence of cysteine protease(s) derived from R bovis, in which the protease(s) would play important roles in the erythrocyte invasion and/or replication processes of the parasite. (c) 2007 Elsevier Inc. All rights reserved.
