Parapusillimonas granuli gen. nov., sp. nov., isolated from granules from a wastewater-treatment bioreactor.
Parapusillimonas granuli gen. nov., sp. nov., isolated from granules from a wastewater-treatment bioreactor.
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颗粒副毛单胞菌属
DOI：
10.1099/ijs.0.009720-0
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发表时间：
2010
影响因子：
2.8
通讯作者：
Deok
中科院分区：
文献类型：
--
作者：
Y. Kim;M. Kim;W. Im;Sathiyaraj Srinivasan;Deok
从韩国一家酒精发酵厂废水处理生物反应器的颗粒中分离到一株新的β-变形杆菌，命名为Ch 07（T）。为了确定其分类学位置，使用多相方法对新菌株进行了表征。新菌株为革兰氏阴性、厌氧、不产孢、短杆状、能动。16 S rRNA基因序列分析表明，菌株Ch 07（T）属于β-变形菌纲，与诺特曼微单胞菌（Pusillimonas noertemannii）BN 9（T）（基因序列相似性为97.30%）、木糖氧化无色杆菌（Achromobacter xylosoxidans subsp.）木糖氧化菌DSM 10346（T）（97.09%）、百日咳博德特氏菌DSM 5571（T）（97.01%）、库氏嗜色素菌DSM 13608（T）（96.68%）和解香卡斯特兰氏菌DSM 1214（T）（96.47%）。DNA-DNA杂交试验结果表明，这些密切相关的模式菌株的重结合值小于62%。化学分类学结果表明，菌株Ch 07（T）具有辅酶Q-8。基因组DNA的G+C含量为67.9+/-0.1mol%。菌株Ch 07（T）的主要多胺为腐胺。菌株Ch 07（T）的极性脂质主要为磷脂酰乙醇胺，其次为二磷脂酰甘油和磷脂酰甘油。当菌株Ch 07（T）在胰蛋白酶大豆琼脂上孵育时，主要的细胞脂肪酸是C（16：0）、C（17：0）环、总和特征3（C（16：1）ω 7 c/iso C（15：0）2-OH）和总和特征5（C（18：1）ω 7 c/ω 9 t/ω 12 t）。DNA-DNA杂交的结果，结合化学分类学和生理学数据，证明菌株Ch 07（T）代表一个新属的新种，其名称为Parapusillimonas granuli gen. nov.，sp. nov.的报道。模式种的模式菌株是Ch 07（T）（=KCTC 12668（T）=LMG 24012（T））。
A novel betaproteobacterium, designated strain Ch07(T), was isolated from granules from the wastewater-treatment bioreactor of an alcohol fermentation factory in South Korea. In order to determine its taxonomic position, the novel strain was characterized using a polyphasic approach. The new strain was Gram-negative, facultatively anaerobic, non-spore-forming, motile and short rod-shaped. 16S rRNA gene sequence analysis revealed that strain Ch07(T) belonged to the class Betaproteobacteria, being related to Pusillimonas noertemannii BN9(T) (gene sequence similarity 97.30 %), Achromobacter xylosoxidans subsp. xylosoxidans DSM 10346(T) (97.09 %), Bordetella pertussis DSM 5571(T) (97.01 %), Pigmentiphaga kullae DSM 13608(T) (96.68 %) and Castellaniella defragrans DSM 1214(T) (96.47 %). The results of DNA-DNA hybridization tests showed that reassociation values were less than 62 % with respect to these closely related type strains. Chemotaxonomic data showed that strain Ch07(T) possessed ubiquinone Q-8. The G+C content of the genomic DNA was 67.9+/-0.1 mol%. The major polyamine of strain Ch07(T) was putrescine. The major polar lipids of strain Ch07(T) were phosphatidylethanolamine, followed by diphosphatidylglycerol and phosphatidylglycerol. When strain Ch07(T) was incubated on tryptic soy agar, the major cellular fatty acids were C(16 : 0), C(17 : 0) cyclo, summed feature 3 (C(16 : 1)omega7c/iso C(15 : 0) 2-OH) and summed feature 5 (C(18 : 1)omega7c/omega9t/omega12t). The results of DNA-DNA hybridizations, in combination with the chemotaxonomic and physiological data, demonstrated that strain Ch07(T) represents a novel species of a new genus, for which the name Parapusillimonas granuli gen. nov., sp. nov. is proposed. The type strain of the type species is Ch07(T) (=KCTC 12668(T)=LMG 24012(T)).