CLINICAL-SIGNIFICANCE OF HUMAN HEPATOCYTE GROWTH-FACTOR IN BLOOD FROM PATIENTS WITH FULMINANT HEPATIC-FAILURE
CLINICAL-SIGNIFICANCE OF HUMAN HEPATOCYTE GROWTH-FACTOR IN BLOOD FROM PATIENTS WITH FULMINANT HEPATIC-FAILURE
复制标题
DOI：
10.1002/hep.1840090615
复制
发表时间：
1989-06-01
期刊：
影响因子：
13.5
通讯作者：
HASHIMOTO, S
中科院分区：
文献类型：
--
作者：
TSUBOUCHI, H;HIRONO, S;HASHIMOTO, S
我们最近在暴发性肝衰竭患者的血清中发现了人肝细胞生长因子的存在，并从一名暴发性肝衰竭患者的血浆中纯化了人肝细胞生长因子。本文报道暴发性肝衰竭患者血液中人肝细胞生长因子的临床意义。采用原代培养的成年大鼠肝细胞，观察了17例暴发性肝衰竭患者血清或血浆对肝细胞生长的影响。17例暴发性肝衰竭患者中有16例的血清或血浆比正常人血清更有效地刺激肝细胞中的DNA合成。17例暴发性肝功能衰竭患者的平均促生长活性约为正常人血清的16倍。患者血液的这种促生长活性与患者的性别、年龄、临床结局、暴发性肝功能衰竭类型无关，但与肝性昏迷的临床分级密切相关。III级和IV级昏迷患者的血清或血浆比II级以下昏迷患者的血清或血浆对DNA合成的刺激活性更明显。在存活组中，这种活性随着患者肝昏迷的改善而降低。事实上，恢复期患者血清的这种活性与正常人血清相比并没有明显增加。在晚期患者组中，这种活动随着昏迷的发展而增加。提示暴发性肝衰竭患者血中人肝细胞生长因子是一种生理性肝营养因子，在患者肝再生中起重要作用。
We have recently found the presence of human hepatocyte growth factor in sera patients with fulminant hepatic failure and have purified human hepatocyte growth factor from plasma of a patient with fulminant hepatic failure. In this paper, we report the clinical significance of human hepatocyte growth factor in blood from patients with fulmiannt hepatic failure. The effect of sera or plasma from 17 patients with fulminant hepatic failure on liver cell growth was examined by use of adult rat hepatocytes in primary culture. Sera or plasma from 16 of the 17 patients with fulminant hepatic failure stimulated DNA synthesis in hepatocytes more effectively than normal human serum. The mean growth-promoting activity for the 17 patients with fulminant hepatic failure was about 16 times higher than that obtained for normal human serum. This growth-promoting acitivity of patients'' blood was not related to sex, age, clinical outcome of the patients or type of fulminant hepatic failure, but was intimately related to the clinical grade of hepatic coma. Sera or plasma with Grade III and IV coma showed stimulatory activity on DNA synthesis more markedly than sera or plasma from patients with coma of less than Grade II. In the surviving group, this activity decreased as the hepatic coma of patients improved. In fact, this acitivy of sera from patients at the recovery stage showed no significant increase compared with that of normal human serum. In the group of terminal patients, this activity increased as the coma developed. These results suggest that human hepatocyte growth factor in blood from patients with fulminant hepatic failure is a physiologicl hepatotrophic factor and plays an important role in liver regeneration of patients.