Human adipsin is identical to complement factor D and is expressed at high levels in adipose tissue.
Human adipsin is identical to complement factor D and is expressed at high levels in adipose tissue.
复制标题
DOI：
10.1016/s0021-9258(19)50409-4
复制
发表时间：
1992-05
期刊：
影响因子：
--
通讯作者：
R. T. White;D. Damm;N. Hancock;B. Rosen;B. Lowell;P. Usher;J. Flier;B. Spiegelman
中科院分区：
文献类型：
--
作者：
R. T. White;D. Damm;N. Hancock;B. Rosen;B. Lowell;P. Usher;J. Flier;B. Spiegelman
分离人adipsin的cDNA，并显示其编码的蛋白质与先前确定的纯化的天然人补体因子D的蛋白质序列具有98%的氨基酸序列相似性。与小鼠脂蛋白酶一样，重组人脂蛋白酶显示人补体因子D的酶活性，仅当B与活化的补体成分C3复合时才切割补体因子B。我们的结论是，人类adipsin相当于补体因子D和adipsin是D因子在啮齿类动物的同系物。脂肪组织是合成人adipsin/补体因子D mRNA的主要部位，但与啮齿动物的情况不同，人adipsin mRNA也在单核细胞/巨噬细胞中表达。这里提供的数据，证明了人adipsin与补体因子D的等效性及其在脂肪中的高水平表达，表明脂肪组织在免疫系统生物学中的先前未被怀疑的作用。
A cDNA for human adipsin was isolated and shown to encode a protein sharing 98% amino acid sequence similarity with the protein sequence previously determined for purified natural human complement factor D. Like mouse adipsin, recombinant human adipsin displays the enzymatic activity of human complement factor D, cleaving complement factor B only when B is complexed with activated complement component C3. We conclude that human adipsin is equivalent to complement factor D and that adipsin is the homologue of factor D in rodents. Adipose tissue is a major site of synthesis of human adipsin/complement factor D mRNA, but unlike the case in rodents, human adipsin mRNA is also expressed in monocytes/macrophages. The data presented here, demonstrating the equivalence of human adipsin to complement factor D and its high level of expression in fat, suggest a previously unsuspected role for adipose tissue in immune system biology.