ACTIN MEMBRANE INTERACTION IN FOCAL ADHESIONS
ACTIN MEMBRANE INTERACTION IN FOCAL ADHESIONS
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DOI：
10.1016/0922-3371(90)90048-2
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发表时间：
1990-12-01
期刊：
影响因子：
--
通讯作者：
TURNER, C
中科院分区：
文献类型：
--
作者：
BURRIDGE, K;NUCKOLLS, G;TURNER, C
粘着斑是质膜的区域，组织培养中的细胞强烈粘附到下面的细胞外基质，并且在其细胞质表面用于锚定肌动蛋白微丝束。 他们为研究细胞骨架和细胞外基质之间的联系提供了一个实验模型。 细胞外基质受体整合素家族的成员是重要的成分，在粘着斑中跨越膜，但有证据表明这些结构的形成还需要其他膜成分。 许多蛋白质集中在粘着斑的细胞质面上。 最近的努力试图确定肌动蛋白和整合素细胞质结构域之间的联系。 使用体外结合测定，已经确定了肌动蛋白和整联蛋白之间的两个潜在桥梁。 其中之一涉及踝蛋白，最近已证明它可以直接结合肌动蛋白。 另一个涉及肌动蛋白结合蛋白α-肌动蛋白，它被发现与几种整合素相互作用。 肌动蛋白和整联蛋白之间这两个潜在桥梁的生理意义仍有待在体内确定。
Focal adhesions are regions of the plasma membrane where cells in tissue culture adhere strongly to the underlying extracellular matrix, and which at their cytoplasmic face serve to anchor bundles of actin microfilaments. They provide an experimental model for studying the links between the cytoskeleton and the extracellular matrix. Members of the integrin family of extracellular matrix receptors are prominent components, spanning the membrane in focal adhesions, but there is evidence that other membrane components are also needed for these structures to form. A number of proteins are concentrated at the cytoplasmic face of focal adhesions. Recent efforts have sought to determine the links between actin and the integrin cytoplasmic domains. Using in vitro binding assays, two potential bridges between actin and integrin have been identified. One involves talin, which has recently been shown to bind actin directly. The other involves the actin-binding protein, alpha-actinin, which has been found to interact with several integrins. The physiological significance of these two potential bridges between actin and integrin remains to be determined in vivo.