Lactobacillus casei CCFM419 attenuates type 2 diabetes via a gut microbiota dependent mechanism.
Lactobacillus casei CCFM419 attenuates type 2 diabetes via a gut microbiota dependent mechanism.
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DOI：
10.1039/c7fo00593h
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发表时间：
2017-09
期刊：
影响因子：
6.1
通讯作者：
Gang Wang;Xiangfei Li;Jianxin Zhao;Hao Zhang;Wei Chen
中科院分区：
文献类型：
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作者：
Gang Wang;Xiangfei Li;Jianxin Zhao;Hao Zhang;Wei Chen
益生菌作为膳食补充剂，通过调节肠道微生物群来传递其主要作用。根据先前的研究，干酪乳杆菌CCFM 419预防糖尿病的一个可能机制可能涉及肠道植物群。为了验证这一假设，高脂肪和链脲佐菌素诱导的C57 BL/6 J小鼠喂养L。在108、109和101（）菌落形成单位（CFU）下的干酪素CCFM 419。与未处理的小鼠相比，109 CFU的L.干酪素CCFM 419减轻了糖尿病的几种症状，包括空腹血糖、餐后血糖、葡萄糖耐受不良和胰岛素抗性。此外，该CFU水平还降低了炎症标志物肿瘤坏死因子-α和白细胞介素-6的水平，并增加了肠胰高血糖素样肽-1（GLP-1）的水平，这与短链脂肪酸（SCFA）的产生有关。粪便样品的16 S rRNA基因测序表明，109 CFU的L. casei CCFM 419显著增加了拟杆菌门的丰度，降低了厚壁菌门的比例，并富集了双歧杆菌、乳杆菌和SCFA产生菌，包括Allobaculum和拟杆菌。这些结果表明L.干酪CCFM 419改变肠道菌群-SCFA-炎症/GLP-1机制以改善2型糖尿病。
Probiotics, as dietary supplements, transmit their major effects through the regulation of gut microbiota. According to a previous study, one possible mechanism of Lactobacillus casei CCFM419 protection against diabetes may involve gut flora. To test this hypothesis, high fat and streptozotocin-induced C57BL/6J mice were fed L. casei CCFM419 at 108, 109, and 1010 colony forming units (CFU). Compared to untreated mice, 109 CFU of L. casei CCFM419 attenuated several symptoms of diabetes, including fasting blood glucose, postprandial blood glucose, glucose intolerance, and insulin resistance. In addition, this CFU level also decreased the levels of the inflammatory markers tumor necrosis factor-α and interleukin-6 and increased intestinal glucagon-like peptide-1 (GLP-1) levels, which are associated with the production of short chain fatty acids (SCFAs). The 16S rRNA gene sequencing of fecal samples demonstrated that 109 CFU of L. casei CCFM419 dramatically increased the abundance of Bacteroidetes and decreased the proportion of Firmicutes at the phylum level, and enriched Bifidobacterium, Lactobacillus, and SCFA-producing bacteria, including Allobaculum and Bacteroides. These findings suggested that L. casei CCFM419 modified the gut flora-SCFA-inflammation/GLP-1 mechanism to ameliorate type 2 diabetes.