Hypoxia-ischemia causes persistent movement deficits in a perinatal rabbit model of cerebral palsy: assessed by a new swim test
Hypoxia-ischemia causes persistent movement deficits in a perinatal rabbit model of cerebral palsy: assessed by a new swim test
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DOI：
10.1016/j.ijdevneu.2009.06.008
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发表时间：
2009-10-01
影响因子：
1.8
通讯作者：
Tan, Sidhartha
中科院分区：
文献类型：
--
作者：
Derrick, Matthew;Drobyshevsky, Alexander;Tan, Sidhartha
在新生儿时期，不同肌肉群之间的运动关系尚未被量化。脑性瘫痪（CP），这往往是由于围产期缺氧缺血（H-1），是根据临床表现，大脑区域的参与和参与程度进行分类。为了测试不同的脑区参与，本研究调查了个体和多关节参与的兔模型CP。妊娠70%的孕兔进行40分钟的子宫缺血。在出生后的前11天内，在5个时间点对新生兔试剂盒进行游泳试验。H-1试剂盒根据出生时的肌张力分为高渗组和非高渗组。测定了支撑游泳过程中前肢和后肢关节（腕、肘、肩、踝、膝和髋）的角运动范围和角运动速度。严重受损（高渗）的动物关节运动的范围和角速度显著降低，并不随时间而改善。非高渗组显示腕关节和后肢运动缺陷，在长期观察中不明显。神经元型一氧化氮合酶抑制剂的预防性治疗降低了严重受损套件的发生率;非高渗套件显示出不同的游泳模式。支撑游泳允许在没有负重的情况下在运动的主平面中量化肢体和关节运动，并减少对平衡控制的需要。鉴定和定量的轻度赤字允许在H-1损伤的原因，以及估计与治疗剂的恢复机制的研究。(C)2009 ISDN。由爱思唯尔有限公司出版。保留所有权利。
The relationship of movement between different muscle groups has not been quantified before in the newborn period. Cerebral palsy (CP), which often occurs as a result of perinatal hypoxia-ischemia (H-1), is categorized depending on clinical presentation, brain region involvement and extent of involvement. In order to test different brain region involvement, this study investigates individual and multi-joint involvement in a rabbit model of CP. Pregnant rabbits at 70% gestation were subjected to 40-min uterine ischemia. Newborn rabbit kits were subjected to a swim test at 5 time points over the first 11 days of life. H-1 kits were divided into hypertonic and non-hypertonic groups based on muscle tone at birth. The ranges and velocity of angular movement of the forelimb and hind limb joints (wrist, elbow, shoulder, ankle, knee and hip) during supported swimming were determined. Severely impaired (hypertonic) animals have significantly reduced range and angular velocity of joint motion, which do not improve over time. The non-hypertonic group showed deficits in wrist and hind limb movements that were not evident on prolonged observation. Preventive treatment with an inhibitor of neuronal nitric oxide synthase decreased the incidence of severely impaired kits; the non-hypertonic kits showed a different pattern of swimming. Supported swimming allows quantification of limb and joint motion in the principal plane of movement in the absence of weight bearing and decreases the need for balance control. Identification and quantification of milder deficits allows mechanistic studies in the causation of H-1 injury as well as estimation of recovery with therapeutic agents. (C) 2009 ISDN. Published by Elsevier Ltd. All rights reserved.