ACID-VOLATILE SELENIUM FORMATION CATALYZED BY GLUTATHIONE REDUCTASE
ACID-VOLATILE SELENIUM FORMATION CATALYZED BY GLUTATHIONE REDUCTASE
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DOI：
10.1021/bi00679a014
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发表时间：
1975-01-01
期刊：
影响因子：
2.9
通讯作者：
GANTHER, HE
中科院分区：
文献类型：
--
作者：
HSIEH, HS;GANTHER, HE
在含20 mM谷胱甘肽、0.05 mM亚硒酸钠、tpnh生成系统和微量高纯度酵母谷胱甘肽还原酶的厌氧系统中，由谷胱甘肽还原酶催化产生酸挥发性硒（p-亲本HySe）。该体系的HySe产量与谷胱甘肽还原酶浓度成正比，在pH为7时达到最大值。在缺乏谷胱甘肽还原酶和TPNH的系统中发生了显著的非酶生成H2Se。浓度的亚砷酸盐（0.1 mM）不抑制谷胱甘肽还原酶抑制硒化物的挥发
The production of acid-volatile selenide (ap-parently HySe) was catalyzed by glutathione reductase in an anaerobic system containing 20 mM glutathione, 0.05 mM sodium selenite, a TPNH-generating system, and microgram quantities of highly purified yeast glutathione re-ductase. HySe production in this system was proportional to glutathione reductase concentration and was maximal at pH 7. Significant nonenzymic H2Se production occurred in the system lacking glutathione reductase and TPNH. A concentration of arsenite (0.1 mM) which does not inhibit glutathione reductase inhibited selenide volatilization, as