Surface recognition of a protein using designed transition metal complexes
Surface recognition of a protein using designed transition metal complexes
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DOI：
10.1021/ja003193z
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发表时间：
2001-07-04
影响因子：
15
通讯作者：
Rodgers, KR
中科院分区：
文献类型：
--
作者：
Fazal, MA;Roy, BC;Rodgers, KR
每种蛋白质在表面上具有独特的组氨酸残基模式。本文介绍了过渡金属配合物的设计，合成和结合研究，以靶向碳酸酐酶（牛红细胞）的表面组氨酸模式。当复合物上的铜离子图案与蛋白质的组氨酸的表面图案相匹配时，可以在水性缓冲液（pH = 7.0）中实现强的和选择性的结合。所描述的蛋白质识别方法适用于已知结构的蛋白质。随着蛋白质结构的快速增长，该方法在蛋白质的纯化、靶向和传感方面具有广泛的适用性。
Each protein has a unique pattern of histidine residues on the surface. This paper describes the design, synthesis, and binding studies of transition metal complexes to target the surface histidine pattern of carbonic anhydrase (bovine erythrocyte). When the pattern of cupric ions on a complex matches the surface pattern of histidines of the protein, strong and selective binding can be achieved in aqueous buffer (pH = 7.0). The described method of protein recognition is applicable to proteins of known structures. With rapidly increasing number of solved protein structures, the method has wide applicability in purification, targeting, and sensing of proteins.