Scanning confocal total internal reflection fluorescence microscopy by using radial polarization in the illumination system
Scanning confocal total internal reflection fluorescence microscopy by using radial polarization in the illumination system
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DOI：
10.1364/ao.48.001114
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发表时间：
2009-02-20
期刊：
影响因子：
1.9
通讯作者：
Kano, Hiroshi
中科院分区：
文献类型：
--
作者：
Terakado, Goro;Watanabe, Kouyou;Kano, Hiroshi
我们报告了一种具有改进的成像性能的扫描全内反射荧光显微镜。在所开发的显微镜的照明系统中，采用径向偏振来获得具有单峰和圆形峰的点扩散函数。通过利用径向偏振，共焦检测方法可以有效地应用于光学系统中，从而降低图像的背景噪声。尺寸为200 nm的荧光颗粒的观察图像揭示了成像性能的改善。 (C) 2009 美国光学学会
We report on a scanning total internal reflection fluorescence microscope with improved imaging properties. In the illumination system of the developed microscope, radial polarization is employed to obtain the point spread function with a single and circular peak. By using radial polarization, the confocal detection method, which can reduce the background noise of the image, is effectively applicable in the optical system. The observed image of a fluorescent particle with the size of 200 nm reveals the improvement of the imaging properties. (C) 2009 Optical Society of America