Laser-driven gel microtool for single-cell manipulation based on temperature control with a photothermal conversion material
Laser-driven gel microtool for single-cell manipulation based on temperature control with a photothermal conversion material
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用于基于光热转换材料温度控制的单细胞操作的激光驱动凝胶微型工具
DOI：
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
F. Arai
中科院分区：
文献类型：
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作者：
T. Hayakawa;M. Kikukawa;H. Maruyama;F. Arai
我们提出了一种激光驱动的混合凝胶微型工具，用于稳定的单细胞操作。该微型工具由用多壁碳纳米管（MWNT）染色的微珠和热敏聚（N-异丙基丙烯酰胺）凝胶涂层制成。凝胶在高温下会粘附在细胞上，但在低温下则不会。我们可以利用凝胶的细胞粘附特性将细胞粘附到微型工具上的凝胶上，从而无需直接激光照射即可操纵单细胞。通过用光镊夹住微型工具来加热，使其表面在操作过程中具有细胞粘附性。此外，我们可以通过用MWNT墨水对微珠进行染色来控制微型工具的光学加热特性。通过改变 MWNT 墨水的浓度，可以将激光加热引起的微型工具温度升高控制在 4.2°C 至 23.5°C 之间。我们成功地制造了所提出的微型工具，并演示了使用微型工具进行单细胞运输，而无需直接激光照射细胞。
We propose a laser-driven hybrid gel microtool for stable single-cell manipulation. The microtool is made of a microbead dyed with multiwalled carbon nanotubes (MWNT) and thermosensitive poly (N-isopropylacrylamide) gel coating. The gel adheres to cells at high temperatures but not at low temperatures. We can manipulate single cells without direct laser irradiation by adhering the cells to the gel on the microtool using the cell-adhesion property of the gel. The microtool is heated by trapping it with optical tweezers to make its surface cell-adhesive during the manipulation. Furthermore, we can control the optical heating property of the microtool by dyeing the microbeads with MWNT ink. The laser-heating-induced temperature increase of the microtool can be controlled from 4.2 °C to 23.5 °C by varying the concentration of MWNT ink. We succeeded in fabricating the proposed microtool and demonstrated single-cell transportation using the microtool without direct laser irradiation of the cell.
影响因子：
10.8
作者：
Richardson HH;Carlson MT;Tandler PJ;Hernandez P;Govorov AO
通讯作者：
Govorov AO