Immunocytochemical studies on the acroframosome during spermiogenesis of the caridean shrimp Macrobrachium nipponense (Crustacea, Natantia)
Immunocytochemical studies on the acroframosome during spermiogenesis of the caridean shrimp Macrobrachium nipponense (Crustacea, Natantia)
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日本沼虾（甲壳纲，Natantia）精子发生过程中顶体的免疫细胞化学研究
DOI：
10.1080/07924259.2010.9652324
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发表时间：
2010-10-01
影响因子：
0.8
通讯作者：
Yang, Wan-Xi
中科院分区：
文献类型：
--
作者：
Li, Zhe;Pan, Chen-Yi;Yang, Wan-Xi
摘要精子发生是雄性生殖细胞分化过程中的一个高度有序的过程。KIFC 1是一种C端驱动蛋白，在哺乳动物精子发生过程中参与顶体形成和核成形。本文利用Western Blot和共聚焦显微镜技术研究了日本沼虾精子发生过程中KIFC 1和微管的存在和分布。KIFC 1首先出现在早期精子细胞的细胞质中，然后以点状模式与顶体共定位。KIFC 1也广泛存在于晚期精子细胞的细胞核中。在中晚期精子细胞中，微管有一个特殊的极化组织，形状像一把外翻的雨伞，我们将这种结构命名为顶框体（AFS）。这种独特的结构由微管构成顶体，这是第一次在无脊椎动物中描述。我们发现，AFS是一种特殊的微管结构，存在于中期和晚期精子细胞的前顶体极; KIFC 1，而不是驱动蛋白1被发现与AFS共定位。我们的结论是，AFS是顶体生物发生的框架和电机KIFC 1参与这些过程。我们认为，AFS可能通过以下方式发挥作用：（1）作为将囊泡运输到原顶体的途径;（2）作为顶体生物发生的框架;（3）作为将顶体拴在核上的锚。
Abstract Spermiogenesis is a highly ordered process in male germ cell differentiation. KIFC1, a C-terminal kinesin, participates in acrosome formation and nuclear shaping during spermiogenesis of mammals. In this paper we used Western Blot and confocal microscopy to identify the existence and distribution of KIFC1 and microtubules during spermiogenesis in the caridean shrimp Macrobrachium nipponense. KIFC1 first appears in the cytoplasm of early spermatids, and then co-localizes with the acrosome in a punctate pattern. KIFC1 is also diffusely present in the nucleus of late spermatids. In mid- and late spermatids, microtubules have a specific polarized organization shaped like an everted umbrella, and we name this structure the acroframosome (AFS). Such a distinct structure formed by microtubules which frames the acrosome is described here for the first time in an invertebrate. We found that the AFS is a special microtubular structure which is present at the proacrosomal pole in mid- and late spermatids; KIFC1 but not kinesin 1 was found to be co-localized with the AFS. Our conclusion is that the AFS is a framework for acrosome biogenesis and the motor KIFC1 participates in these processes. We propose that the AFS may function in the following ways: (1) as a pathway to transport vesicles to the proacrosome; (2) as a framework for acrosome biogenesis; (3) as an anchor to tether the acrosome to the nucleus.