A universal adhesive incorporating antimicrobial peptide nisin: effects on Streptococcus mutans and saliva-derived multispecies biofilms
A universal adhesive incorporating antimicrobial peptide nisin: effects on Streptococcus mutans and saliva-derived multispecies biofilms
复制标题
含有抗菌肽乳链菌肽的通用粘合剂：对变形链球菌和唾液来源的多物种生物膜的影响
DOI：
10.1007/s10266-019-00478-8
复制
发表时间：
2020-07-01
期刊：
影响因子：
2.5
通讯作者：
Gu，Lisha
中科院分区：
文献类型：
--
作者：
Zhao，Manduo;Qu，Yang;Gu，Lisha
为提高通用型胶粘剂的抗菌活性，将抗菌肽nisin引入到SingleBond Universal中，研究其对变形链球菌单特异性生物膜和唾液来源的多物种生物膜的抗菌作用。将乳酸链球菌素（Nisin）与单键通用（SingleBond Universal）复合，采用激光共聚焦显微镜（CLSM）、逆转录-定量聚合酶链反应（qRT-PCR）、苯酚-硫酸法和乳酸脱氢酶法检测Nisin的抗菌活性。通过微拉伸强度（μTBS）和转化率（DC）测试了复合材料的粘接性能。资料分析采用单因素方差分析和最小显著性差异多重比较检验（P < 0.05）。添加3%（w/v）乳酸链球菌素的单键通用能显著抑制S.与胞外多糖（EPS）合成相关基因（gtfB、gtfC、gtfD和spaP）和产酸相关基因（ldh）的表达显著降低（P < 0.05）。添加3%（w/v）Nisin的SingleBond Universal也能抑制唾液来源的多物种生物膜的生长，并减少EPS和乳酸的分泌（P< 0.05）。加入3%（w/v）nisin的SingleBond Universal组的μTBS和DC无明显恶化（P > 0.05）。总之，3%（w/v）乳酸链球菌素掺入的单键通用基本上抑制了两种S.变形单特异性和唾液衍生的多物种生物膜，而不损害结合性能。
For purpose of enhancing the antibacterial activity of a universal adhesive, the antimicrobial peptide nisin was incorporated into Single Bond Universal and its antibacterial effect on Streptococcus mutans monospecific biofilms and saliva-derived multispecies biofilms was studied. Nisin was incorporated into Single Bond Universal and the antibacterial activity was examined by confocal laser scanning microscopy (CLSM), reverse transcription-quantitative polymerase chain reaction (qRT-PCR), phenol-sulfuric acid method and lactate dehydrogenase enzymatic method. The bonding properties were tested by microtensile bond strength (μTBS) and degree of conversion (DC). Data were analyzed by one-way analysis of variance (ANOVA) and least significant difference multiple comparison tests (P < 0.05). The Single Bond Universal incorporated with 3% (w/v) nisin could significantly inhibit the growth of the S. mutans monospecific biofilms (P< 0.01) and decrease the expression of genes related to extracellular polysaccharide (EPS) synthesis (gtfB, gtfC, gtfD and spaP) and acidogenicity (ldh) (P < 0.05). 3% (w/v) nisin-incorporated Single Bond Universal could also inhibit the growth of saliva-derived multispecies biofilms and decrease the excretion of EPS and lactic acid ( P< 0.05). μTBS and DC of 3% (w/v) nisin-incorporated Single Bond Universal did not deteriorate obviously (P > 0.05). In conclusion, 3% (w/v) nisin-incorporated Single Bond Universal substantially inhibited the growth of both S. mutans monospecific and saliva-derived multispecies biofilms without compromising the bonding properties.