Pseudomonas songnenensis sp nov., isolated from saline and alkaline soils in Songnen Plain, China
Pseudomonas songnenensis sp nov., isolated from saline and alkaline soils in Songnen Plain, China
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Pseudomonas sonnenensis sp nov.，从中国松嫩平原盐碱土中分离出来
DOI：
10.1007/s10482-014-0365-3
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发表时间：
2015
影响因子：
2.6
通讯作者：
Jiang Juquan
中科院分区：
文献类型：
--
作者：
Zhang Lei;Pan Yuanyuan;Wang Kaibiao;Zhang Xiaoxia;Zhang Shuang;Fu Xiaowei;Zhang Cheng;Jiang Juquan
菌株neau - st5 - 5t从松嫩平原盐碱地中分离得到。该细菌为好氧菌，革兰氏染色阴性，杆状，通过几个极性鞭毛运动。它形成黄橙色的菌落，表面呈放射状褶皱。根据分离的16S rRNA基因序列和连接的16S rRNA、gyrBandrpoDgene序列进行系统发育分析，表明该菌株属于gammaproteobacteria纲假单胞菌属。菌株neau - st5 - 5t16s rRNA与假单胞菌近缘种型菌株的基因序列相似性分别为98.8% ~ 97.1%、90.5% ~ 78.4%、90.4% ~ 71.1%。菌株neau - st5 - 5t与最近亲缘种假单胞菌stutzeriDSM 5190T，P。xanthomarinaDSM 18231 t, P。kunmingensisCGMCC 1.12273 t, P。alcaliphilaDSM 17744 tandp。oleovoranssubsp。润滑剂sdsm21016t为43±1 ~ 25±2%。主要脂肪酸（bbb10 %）为C18:1ω7c/C18:1ω6c、C16:1ω7c/C16:1ω6c和C16:0，主要呼吸醌为ubiquin酮9，极性脂质由磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、一种未知磷脂、一种未知氨基磷脂和一种未知脂质组成。基因型、化学分类和表型分析表明，菌株neau - st5 -5是假单胞菌属的一种新种，命名为epseudomonas songnenensis。11月被提议。型应变为NEAU-ST5-5T（=ACCC 06361T= DSM 27560T）。
The strain NEAU-ST5-5Twas isolated from the saline and alkaline soil in Songnen Plain, North East of China. The bacterium was found to be aerobic, Gram-stain negative, rod-shaped and motile by means of several polar flagella. It forms yellow-orange colonies with a radial wrinkled surface. Phylogenetic analyses based on the separate 16S rRNA gene sequences and concatenated 16S rRNA,gyrBandrpoDgene sequences indicated that it belongs to the genusPseudomonasin the classGammaproteobacteria. Strain NEAU-ST5-5Tshows gene sequence similarities of 98.8–97.1 % for 16S rRNA, 90.5–78.4 % forgyrBand 90.4–71.1 % forrpoDwith type strains of the closely related species of the genusPseudomonas, respectively. DNA–DNA hybridization relatedness between strain NEAU-ST5-5Tand type strains of the most closely related species,Pseudomonas stutzeriDSM 5190T,P. xanthomarinaDSM 18231T,P. kunmingensisCGMCC 1.12273T,P. alcaliphilaDSM 17744TandP. oleovoranssubsp.lubricantisDSM 21016Twere 43 ± 1 to 25 ± 2 %. The major fatty acids (>10 %) were determined to be C18:1ω7c/C18:1ω6c, C16:1ω7c/C16:1ω6c and C16:0, the predominant respiratory quinone was identified as ubiquinone 9 and polar lipids were found to consist of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unknown phospholipid, one unidentified aminophospholipid and one unknown lipid. The genotypic, chemotaxonomic and phenotypic analysis indicated that strain NEAU-ST5-5Trepresents a novel species of the genusPseudomonas, for which the namePseudomonas songnenensissp. nov. is proposed. The type strain is NEAU-ST5-5T(=ACCC 06361T= DSM 27560T).