MOLECULAR CHARACTERIZATION OF NSCL, A GENE ENCODING A HELIX LOOP HELIX PROTEIN EXPRESSED IN THE DEVELOPING NERVOUS-SYSTEM
MOLECULAR CHARACTERIZATION OF NSCL, A GENE ENCODING A HELIX LOOP HELIX PROTEIN EXPRESSED IN THE DEVELOPING NERVOUS-SYSTEM
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DOI：
10.1073/pnas.89.1.38
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发表时间：
1992-01-01
影响因子：
11.1
通讯作者：
KIRSCH, IR
中科院分区：
文献类型：
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作者：
BEGLEY, CG;LIPKOWITZ, S;KIRSCH, IR
我们在这里报道了螺旋-环-螺旋（HLH）转录因子家族的另一个成员NSCL的分子克隆和染色体定位。NSCL基因是根据其与先前描述的造血HLH基因SCL的杂交鉴定的。小鼠NSCL cDNA克隆从第11.5天的小鼠胚胎cDNA文库中获得。编码区为399个碱基对，预计编码14.8 kDa的蛋白。在HLH结构域，与小鼠SCL的核苷酸序列和氨基酸序列的同源性分别为71%和61%。NSCL蛋白编码区在HLH结构域的第二个两亲螺旋外终止6个氨基酸。在交配后9.5 ~ 14.5天的小鼠胚胎RNA中检测到NSCL的表达，在10.5 ~ 12天达到最高表达水平。通过原位杂交检查12天和13天的小鼠胚胎，发现NSCL在发育中的神经系统中表达。将nsl基因定位到小鼠1号染色体上。非鳞状细胞白血病非常有限的表达模式表明这种HLH蛋白在神经发育中起重要作用。
We report here the molecular cloning and chromosomal localization of an additional member of the helix-loop-helix (HLH) family of transcription factors, NSCL. The NSCL gene was identified based on its hybridization to the previously described hemopoietic HLH gene, SCL. Murine NSCL cDNA clones were obtained from a day 11.5 mouse embryo cDNA library. The coding region is 399 base pairs and encodes a predicted protein of 14.8 kDa. The nucleotide sequence shows 71% identity and the amino acid sequence shows 61% identity to murine SCL in the HLH domain. The NSCL protein-coding region terminates six amino acids beyond the second amphipathic helix of the HLH domain. Expression of NSCL was detected in RNA from mouse embryos between 9.5 and 14.5 days postcoitus, with maximum levels of expression at 10.5-12 days. Examination of 12- and 13-day mouse embryos by in situ hybridization revealed expression of NSCL in the developing nervous system. The NSCL gene was mapped to murine chromosome 1. The very restricted pattern of NSCL expression suggests an important role for this HLH protein in neurological development.