Exploring the hidden human urinary proteome via ligand library beads
Exploring the hidden human urinary proteome via ligand library beads
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DOI：
10.1021/pr050153r
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发表时间：
2005-11-01
影响因子：
4.4
通讯作者：
Rigetti, PG
中科院分区：
文献类型：
--
作者：
Castagna, A;Cecconi, D;Rigetti, PG
人尿蛋白质组已被重新评估和重新评估，通过一种新的浓缩/均衡技术，利用珠包被六聚体肽配体库。这些珠子通过捕获样品中包含的整个蛋白质光谱，通过大幅降低最丰富的物种的水平，同时强烈浓缩更稀和稀有的物种。在对照尿样中，可以鉴定出134种独特的蛋白质。第一珠洗脱液（在硫脲、尿素和CHAPS中）允许鉴定317种基因产物，而第二洗脱液（在9 M尿素中，pH 3.8）允许鉴定另外95种独特的蛋白质。通过消除冗余，总共可以在人类尿液中识别出383种独特的基因产物。与最近文献中报告的数据相比，这是一个主要的增量。通过将我们的数据与目前报道的数据进行比较，可以将另外251种蛋白质添加到列表中，从而使迄今为止在人类尿液中鉴定的独特基因产物总数达到约800种。
The human urinary proteome has been reassessed and re-evaluated via a novel concentration/equalization technique, exploiting beads coated with hexameric peptide ligand libraries. These beads act by capturing the whole protein spectra contained in the sample, by drastically reducing the level of the most abundant species, while strongly concentrating the more dilute and rare ones. In a control urine sample, 134 unique proteins could be identified. The first bead eluate (in thiourea, urea, and CHAPS) permitted the identification of 317 gene products, whereas the second eluale (in 9 M urea, pH 3.8) allowed the identification of another 95 unique proteins. By eliminating redundancies, a total of 383 unique gene products could be identified in human urines. This represents a major increment as compared to data reported in recent literature. By comparing our data with those reported to the present, an additional 251 proteins could be added to the list, thus bringing the total unique gene products so far identified in human urines to ca.800 species.