MicroRNA regulation of cyclooxygenase-2 during embryo implantation
MicroRNA regulation of cyclooxygenase-2 during embryo implantation
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DOI：
10.1073/pnas.0705917104
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发表时间：
2007-09-18
影响因子：
11.1
通讯作者：
Dey, Sudhansu K.
中科院分区：
文献类型：
--
作者：
Chakrabarty, Anindita;Tranguch, Susanne;Dey, Sudhansu K.
着床过程是复杂的，需要有发育能力的囊胚和接受子宫之间的相互作用。由于microRNA（miRNAs）在调控基因表达中起主要作用，我们推测它们参与指导植入过程中高度调控的时空表达遗传网络。在这里，我们发现两种miRNAs，mmu-miR-101 a和mmu-miR-199 a *，在着床过程中在小鼠子宫中时空表达，与环氧化酶-2的表达一致，环氧化酶-2是着床的关键基因。更有趣的是，我们的体外功能获得和丧失实验表明，环氧合酶-2的表达是由这两种miRNA转录后调节的。我们报道了在着床过程中miRNA介导的子宫基因表达调控。我们相信，许多与这一过程相关的其他关键基因也受到miRNAs的调控。因此，阐明子宫miRNAs的生理作用将有助于我们更好地了解植入的遗传控制，这是成功怀孕的门户。
The implantation process is complex, requiring reciprocal interactions between implantation-competent blastocysts and the receptive uterus. Because microRNAs (miRNAs) have major roles in regulating gene expression, we speculated that they participate in directing the highly regulated spatiotemporally expressed genetic network during implantation. Here, we show that two miRNAs, mmu-miR-101a and mmu-miR-199a*, are spatiotemporally expressed in the mouse uterus during implantation coincident with expression of cyclooxygenase-2, a gene critical for implantation. More interestingly, our in vitro gain- and loss-of-function experiments show that cyclooxygenase-2 expression is posttranscriptionally regulated by these two miRNAs. We report on miRNA-mediated regulation of uterine gene expression in the context of implantation. We believe that many other critical genes related to this process are also regulated by miRNAs. Thus, elucidating the physiological roles of uterine miRNAs will help us better understand the genetic control of implantation, the gateway to a successful pregnancy.