Global identification of human transcribed sequences with genome tiling arrays
Global identification of human transcribed sequences with genome tiling arrays
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DOI：
10.1126/science.1103388
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发表时间：
2004-12-24
期刊：
影响因子：
56.9
通讯作者：
Snyder, M
中科院分区：
文献类型：
--
作者：
Bertone, P;Stolc, V;Snyder, M
阐明基因组的转录区域构成了人类生物学的基本方面，但这仍然是一个杰出的问题。为了全面识别编码序列，我们构建了一系列高密度寡核苷酸瓷砖阵列，代表了人类基因组的整个非反复序列的感觉和反义链。转录的序列通过杂交与互补的DNA样品跨基因组，从从人肝组织获得的聚腺苷酸化RNA进行了反转录。除了确定许多已知和预测的基因外，我们还发现了10,595个未被其他方法检测到的转录序列。其中很大一部分位于远端基因间区域。从先前注释的基因和其他哺乳动物蛋白具有重要同源性。
Elucidating the transcribed regions of the genome constitutes a fundamental aspect of human biology, yet this remains an outstanding problem. To comprehensively identify coding sequences, we constructed a series of high-density oligonucleotide tiling arrays representing sense and antisense strands of the entire nonrepetitive sequence of the human genome. Transcribed sequences were located across the genome via hybridization to complementary DNA samples, reverse-transcribed from polyadenylated RNA obtained from human liver tissue. In addition to identifying many known and predicted genes, we found 10,595 transcribed sequences not detected by other methods. A large fraction of these are located in intergenic regions distal. from previously annotated genes and exhibit significant, homology to other mammalian proteins.