Mutual Cross Talk between the Regulators Hac1 of the Unfolded Protein Response and Gcn4 of the General Amino Acid Control of Saccharomyces cerevisiae
Mutual Cross Talk between the Regulators Hac1 of the Unfolded Protein Response and Gcn4 of the General Amino Acid Control of Saccharomyces cerevisiae
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DOI：
10.1128/ec.00123-13
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发表时间：
2013-08-01
期刊：
影响因子：
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通讯作者：
Braus, Gerhard H.
中科院分区：
文献类型：
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作者：
Herzog, Britta;Popova, Blagovesta;Braus, Gerhard H.
Hac1是细胞对内质网中未折叠蛋白积聚的反应的激活剂。Hac1的功能需要Gcn4的活性，Gcn4主要作为一般氨基酸控制网络的调节器，为酿酒酵母细胞提供氨基酸。在这里，我们展示了Hac1的新功能，并描述了Hac1和Gcn4之间的相互联系。Hac1是在单倍体和二倍体细胞中诱导Gcn4应答启动子元件所必需的，因此参与了细胞氨基酸的供应。此外，Hac1和Gcn4相互影响其mRNA的表达水平。Hac1还参与了FLO11的表达和对氨基酸饥饿的黏附。Hac1和Gcn4通过FLO11絮凝素的相同启动子区域起作用。结果表明，这两种转录因子对FLO11的表达有间接影响。我们的数据表明，Hac1和Gcn4控制的网络之间存在复杂的相互干扰。
Hac1 is the activator of the cellular response to the accumulation of unfolded proteins in the endoplasmic reticulum. Hac1 function requires the activity of Gcn4, which mainly acts as a regulator of the general amino acid control network providing Saccharomyces cerevisiae cells with amino acids. Here, we demonstrate novel functions of Hac1 and describe a mutual connection between Hac1 and Gcn4. Hac1 is required for induction of Gcn4-responsive promoter elements in haploid as well as diploid cells and therefore participates in the cellular amino acid supply. Furthermore, Hac1 and Gcn4 mutually influence their mRNA expression levels. Hac1 is also involved in FLO11 expression and adhesion upon amino acid starvation. Hac1 and Gcn4 act through the same promoter regions of the FLO11 flocculin. The results indicate an indirect effect of both transcription factors on FLO11 expression. Our data suggest a complex mutual cross talk between the Hac1- and Gcn4-controlled networks.