Orthogonal Surface Acoustic Wave (SAW) Sensor for Cancer Biomarker Detection with Accelerated Binding Kinetics
Orthogonal Surface Acoustic Wave (SAW) Sensor for Cancer Biomarker Detection with Accelerated Binding Kinetics
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DOI：
10.1109/sensors52175.2022.9967354
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发表时间：
2022-10
期刊：
影响因子：
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通讯作者：
Yuqi Huang;Maëlys Boucher;T. Evans-Nguyen;V. Bhethanabotla
中科院分区：
文献类型：
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作者：
Yuqi Huang;Maëlys Boucher;T. Evans-Nguyen;V. Bhethanabotla
在st -石英衬底上制作了一种结合瑞利波和剪切水平表面声波的装置。瑞利波诱导的微流体混合对加速抗体与抗原的实时传感结合动力学有影响，这是通过st -石英上剪切水平表面声波方向的相位变化来测量的。初步实验结果表明，瑞利波微流加速结合时，反应时间缩短，相信号增强。该设备可以使用低成本的单步光刻方法制造，并且可以与用于数据读取的小型电子传感器相结合，从而允许在便携式平台上进行各种基于表面的生物标志物检测。在这项工作中，研究了癌胚抗原（CEA）与功能化捕获抗体结合的检测，以显示瑞利波微流体流的质量负载扩增的影响。
A device incorporating both Rayleigh wave and shear horizontal surface acoustic waves is made on a ST-Quartz substrate. The Rayleigh wave induced microfluidic mixing shows effects on accelerating the binding kinetics of real-time sensing between antibody and antigen, which is measured by phase change from the shear horizontal surface acoustic wave direction on the ST-Quartz. Preliminary results on this device show shortened response time and enhanced phase signal when the binding is accelerated by microfluidic streaming from the Rayleigh wave. The device can be fabricated using a low cost, single step photolithography method and can be combined with a small electronic sensor for data readout, which allows for a variety of surface-based biomarker detections on a portable platform. In this work, detection of Carcinoembryonic antigen (CEA) binding with functionalized capture antibody is studied to show the effects of mass loading amplification due to Rayleigh wave microfluidic streaming.