BEHAVIOR OF SPINDLES AND SPINDLE PLAQUES IN CELL-CYCLE AND CONJUGATION OF SACCHAROMYCES-CEREVISIAE
BEHAVIOR OF SPINDLES AND SPINDLE PLAQUES IN CELL-CYCLE AND CONJUGATION OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/jb.124.1.511-523.1975
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发表时间：
1975-01-01
影响因子：
3.2
通讯作者：
GOETSCH, L
中科院分区：
文献类型：
--
作者：
BYERS, B;GOETSCH, L
在酿酒酵母中，纺锤体空斑与细胞分裂和接合的其他方面的相互依赖性已经被研究。在完整的核内纺锤体形成之前，依次出现三种形式的纺锤体斑块。单个空斑最初出现在有丝分裂周期中，在G1后期转变为携带卫星的单个空斑。随后，空斑复制产生出芽早期的双空斑特征，这与染色体复制期（S）一致。这些噬菌斑最终分离形成一个完整的纺锤体，在每一极都有一个噬菌斑，这几乎与S.斑块的形式不同，在两个独立的情况下，G1逮捕：单一的斑块被发现在一个细胞中静止逮捕的增长，而一个细胞逮捕的交配因素在准备共轭包含一个卫星轴承单一的斑块。后一种形式在合子形成期间被保留，在那里它作为每个前合子核与另一个核融合的初始位点。这种融合的结果是形成一个单一的合子核与卫星轴承单一的斑块，这是随后转化为双斑作为合子芽。在营养细胞和合子中，双空斑都位于芽出现的部位附近。
The interdependence of spindle plaque with other aspects of cell division and conjugation in Saccharomyces cerevisiae has been investigated. Three forms of the spindle plaque appear sequentially before the formation of the complete, intranuclear spindle. The single plaque is present initially in the mitotic cycle; it becomes transformed into a satellite-bearing single plaque during the latter part of G1. Subsequently, plaque duplication yields the double plaque characteristic of the early phase of budding, which coincides with the period of chromosome replication (S). The eventual separation of these plaques to form a complete spindle, with a single plaque at each pole, is nearly coincident with the completion of S. The form of the plaque differs in two independent cases of G1 arrest: the single plaque is found in a cell in stationary arrest of growth, whereas a cell arrested by mating factors in preparation for conjugation contains a satellite-bearing single plaque. The latter form is retained during zygote formation, where it serves as the initial site of fusion of each prezygotic nuceus with the other. This fusion results in the formation of a single zygotic nucleus with a satellite-bearing single plaque, which is subsequently transformed into a double plaque as the zygote buds. The double plaque is situated adjacent to the site of bud emergence in both vegetative cells and zygotes.