Sunlight Inactivation of Human Polymerase Chain Reaction Markers and Cultured Fecal Indicators in River and Saline Waters
Sunlight Inactivation of Human Polymerase Chain Reaction Markers and Cultured Fecal Indicators in River and Saline Waters
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DOI：
10.2175/106143012x13560205144290
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发表时间：
2013-08-01
影响因子：
3.1
通讯作者：
Sinton, Lester W.
中科院分区：
文献类型：
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作者：
Gilpin, Brent J.;Devane, Megan;Sinton, Lester W.
总拟杆菌目、人类特异性拟杆菌目、大肠杆菌和双歧杆菌相对于培养的大肠杆菌的聚合酶链反应（PCR）标记在阳光下失活的衰减率。大肠杆菌中进行了研究。该实验使用100升的淡水和海水（与黑暗对照配对），用人类污水播种，并暴露在自然阳光下三个夏天。可培养E.在第1天，暴露于阳光下的室中的大肠杆菌水平降低了至少3个对数，并且到第3天，在淡水和海水中分别实现了4.5至5.5个对数的总共降低。相比之下，在研究期间，日光暴露室中四种基因靶的PCR检测减少不超过2个log（k（t）< 0.071 log（e）单位h（-1））。在这些实验条件下，PCR标记比可培养的E.大肠杆菌，并可以持续很长一段时间后，灭活的E。杆菌
Decay rates for sunlight inactivation of polymerase chain reaction (PCR) markers for total Bacteroidales, human-specific Bacteroidales, Escherichia coli, and Bifidobacterium adolescentis relative to cultured E. coli were investigated. The experiment used 100-L chambers of fresh water and seawater (paired with dark controls) seeded with human sewage and exposed to natural sunlight over three summer days. Culturable E. coli levels in sunlight-exposed chambers decreased by at least 3 logs on day 1, and by day 3 a total reduction of 4.5 to 5.5 logs was achieved in fresh water and seawater, respectively. In contrast, PCR detection of the four gene targets in sunlight-exposed chambers reduced by no more than 2 logs over the duration of the study (k(t) < 0.071 log(e) units h(-1)). Under these experimental conditions, PCR markers are considerably more conservative than culturable E. coli and can persist for extended periods of time following inactivation of E. coli.