Postnatal expression of the plasticity-related nerve growth factor-induced gene A (NGFI-A) protein in the superficial layers of the rat superior colliculus:: Relation to N-methyl-D-aspartate receptor function
Postnatal expression of the plasticity-related nerve growth factor-induced gene A (NGFI-A) protein in the superficial layers of the rat superior colliculus:: Relation to N-methyl-D-aspartate receptor function
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DOI：
10.1016/j.neuroscience.2004.08.004
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Mendez-Otero, R
中科院分区：
文献类型：
--
作者：
Giraldi-Guimaraes, A;De Bittencourt-Navarrete, RE;Mendez-Otero, R
中枢神经系统中的早期基因表达是由感觉刺激诱导的，并且似乎与长期突触可塑性有关。我们采用免疫组化方法检测出生后发育过程中大鼠上丘浅层神经生长因子诱导基因A（NGFI-A）蛋白的表达。我们的目标是将这种候选可塑性蛋白的表达与发育事件相关联，特别是视网膜丘脑和皮质丘脑途径的活动依赖性细化。我们还研究了 NGFI-A 表达的 N-甲基-D-天冬氨酸 (NMDA) 受体依赖性。使用不同产后年龄的动物。出生后第 12 天和年龄较大的动物接受暗适应方案，然后进行光刺激。在出生后的前 2 周内从未观察到 NGFI-A 表达。第一个染色细胞在 P15 时观察到，即睁眼后 2 天（P13）。在出生后第三周结束时观察到染色细胞数量最高（P22）。在 P30 时达到了类似成人的表达水平，因为在这个年龄，染色细胞的数量与成年大鼠 (P90) 中发现的细胞数量相当。接受 MK-801（腹腔注射）急性治疗的 P22 动物和接受 MK-801 慢性颅内输注的成年动物均表现出响应光刺激的 NGFI-A 表达明显下降。这些结果表明，NGFI-A 的表达依赖于 NMDA 受体的激活，并且观察到的表达模式与之前对发育中的大鼠上丘 (SC) 中 NMDA 受体介导的视觉活动变化的描述非常一致。我们的结果表明，可塑性相关的 NGFI-A 蛋白可能在大鼠睁眼后 SC 浅层的发育可塑性中发挥作用。 (C) 2004 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
Immediate early gene expression in the CNS is induced by sensory stimulation and seems to be involved in long-term synaptic plasticity. We have used an immunohistochemical method to detect the nerve growth factor-induced gene A (NGFI-A) protein expression in the superficial layers of the rat superior colliculus during postnatal development. Our goal was to correlate the expression of this candidate plasticity protein with developmental events, especially the activity-dependent refinement of the retinocollicular and corticocollicular pathways. We have also investigated the N-methyl-D-aspartate (NMDA)-receptor dependence of the NGFI-A expression. Animals of various postnatal ages were used. Postnatal day (P) 12 and older animals were submitted to a protocol of dark adaptation followed by light stimulation. NGFI-A expression was never observed during the first 2 postnatal weeks. The first stained cells were observed at P15, 2 days after eye opening (P13). The highest number of stained cells was observed at the end of the third postnatal week (P22). Adult-like level of expression was reached at P30, since at this age, the number of stained cells was comparable to that found in adult rats (P90). Both P22 animals submitted to an acute treatment with MK-801 (i.p. injection) and adult animals submitted to chronic intracranial infusion of a MK-801 presented a clear decrease in the NGFI-A expression in response to light stimulation. These results suggest that the NGFI-A expression is dependent on the NMDA receptor activation, and the observed pattern of expression is in close agreement with previous descriptions of the changes in the NMDA receptor-mediated visual activity in the developing rat superior colliculus (SC). Our results suggest that the plasticity-related NGFI-A protein might play a role in the developmental plasticity of the superficial layers of the rat SC after eye opening. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.