Msx1 regulates proliferation and differentiation of mouse dental mesenchymal cells in culture
Msx1 regulates proliferation and differentiation of mouse dental mesenchymal cells in culture
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DOI：
10.1111/eos.12078
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发表时间：
2013-10-01
影响因子：
1.9
通讯作者：
Ge, Li-hong
中科院分区：
文献类型：
--
作者：
Feng, Xiao-yu;Zhao, Yu-ming;Ge, Li-hong
同源框 msh 样 1 (MSX1) 蛋白对于细胞增殖和分化至关重要。 Msx1 敲除小鼠的牙胚发育在萌芽阶段被抑制，这阻碍了对其在牙齿发育这一阶段之后的作用的理解。本研究旨在探讨MSX1在调节培养的牙本质间充质细胞增殖和分化中的潜在作用，并初步探讨其潜在作用机制。从胚胎日 (E)15.5 小鼠中分离出牙胚。分离间充质并消化成单细胞悬液，然后进行体外培养。将MSX1小干扰RNA转染分离的牙体间充质细胞，研究其对细胞增殖、细胞周期分布以及骨形态发生蛋白2（Bmp2）和骨形态发生蛋白4（Bmp4）表达的影响。我们还比较了诱导成牙本质细胞分化后对照组和 MSX1 siRNA 转染组之间碱性磷酸酶 (Alp)、I 型胶原 (Col1A)、骨钙素 (Ocn)、runt 相关转录因子 2 (Runx2)、牙本质唾液酸磷蛋白 (Dspp) 和牙本质基质蛋白 1 (Dmp1) 的表达水平以及矿化结节的形成。 Msx1 表达的敲低与细胞增殖减少、细胞周期 S 期时间延长、成牙本质细胞分化增强以及 Bmp2 和 Bmp4 表达升高相关。我们得出结论，MSX1可能通过抑制Bmp2和Bmp4的表达来促进牙间充质细胞的增殖并阻止其分化。
The homeobox, msh-like 1 (MSX1) protein is essential for cell proliferation and differentiation. Tooth germ development of Msx1 knockout mouse is arrested at the bud stage, impeding an understanding of its role beyond this stage of tooth development. The aims of this study were to investigate the potential role of MSX1 in the regulation of proliferation and differentiation of dental mesenchymal cells in culture, and to preliminarily explore its underlying mechanism of action. Tooth germs were isolated from embryonic day (E)15.5 mice. The mesenchyme was separated and digested into a single-cell suspension, and then cultured in vitro. Isolated dental mesenchymal cells were transfected with MSX1 small interfering RNA, and the effects on cell proliferation, cell cycle distribution, and the expression of bone morphogenetic protein 2 (Bmp2) and bone morphogenetic protein 4 (Bmp4) were studied. We also compared the expression levels of alkaline phosphatase (Alp), type I collagen (Col1A), osteocalcin (Ocn), runt-related transcription factor 2 (Runx2), dentin sialophosphoprotein (Dspp) and dentin matrix protein 1 (Dmp1), and mineralized nodule formation, between control and MSX1 siRNA-transfected groups after the induction of odontoblast differentiation. Knockdown of Msx1 expression was associated with decreased cell proliferation, prolonged time in the S phase of the cell cycle, enhanced odontoblast differentiation, and elevated Bmp2 and Bmp4 expression. We conclude that MSX1 may promote proliferation and prevent the differentiation of dental mesenchymal cells by the inhibition of Bmp2 and Bmp4 expression.