Methods of split-reporter reconstitution for the analysis of biomolecules.
Methods of split-reporter reconstitution for the analysis of biomolecules.
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用于生物分子分析的分裂报告基因重建方法。
DOI：
10.1002/tcr.201402001
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
H. Yoshimura and T. Ozawa
中科院分区：
文献类型：
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作者：
高橋恒太;黒澤昌志;池上浩;柴山茂久;坂下満男;竹内和歌奈;中塚理;財満鎭明;S. Yokoyama;K. Itano and T. Iizuka.;H. Yoshimura and T. Ozawa
活细胞内的细胞内信号是由包括蛋白质在内的生物分子的特定定位控制的，其时间尺度从几毫秒到几个小时不等。为了阐明相关的空间和时间信号过程，开发用于细胞事件的光学探针是目前研究的一个具有挑战性的任务。在此，我们描述了受发光生物启发的光学探针基本设计的最新进展，以及它们在活细胞和动物中可视化细胞内事件的实际应用。对不同探针设计的讨论揭示了它们的优点和缺点。
Intracellular signaling inside living cells is controlled by the specific localization of biomolecules, including proteins, with timescales ranging from milliseconds to several hours. To elucidate the related spatial and temporal signal processes, development of optical probes for cellular events is a challenging task in present studies. Herein, we describe recent advances in the basic design of the optical probes, which have been inspired by luminescent creatures, and their practical application to visualize intracellular events in living cells and animals. A discussion of different probe designs reveals their benefits and shortcomings.