Preparation and recognition performance of cholic acid-imprinted material prepared with novel surface-imprinting technique
Preparation and recognition performance of cholic acid-imprinted material prepared with novel surface-imprinting technique
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DOI：
10.1016/j.polymer.2009.05.008
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发表时间：
2009-07-03
期刊：
影响因子：
4.6
通讯作者：
Guo, Jinfeng
中科院分区：
文献类型：
--
作者：
Gao, Baojiao;Lu, Jinhua;Guo, Jinfeng
通过铈盐引发聚丙烯酰胺（AM）在交联聚乙烯醇（CPVA）微球表面接枝聚合，接枝后的聚丙烯酰胺（PAM）经Hofmann降解反应转化为聚乙烯胺（PVAm），得到接枝微球PVAm/CPVA。采用我们提出的新型表面分子印迹技术，以戊二醛为交联剂制备了胆酸分子印迹材料MIP-PVAm/CPVA，并以化学结构与胆酸有一定相似的胆固醇为对照物，采用批法和柱法深入研究了MIP-PVAm/CPVA对胆酸分子的结合特性。实验结果表明，表面印迹材料MIP-PVAm/CPVA对模板分子胆酸具有良好的结合亲和力和识别选择性。PVAm/CPVA微球（非印迹材料）对胆酸相对于胆固醇的选择性系数仅为1.314，对胆酸的识别选择性很差。而印迹后，MIP-PVAm/CPVA对胆酸对胆固醇的选择性系数显著提高至11.231，显示出对胆酸分子极好的识别选择性和结合亲和力。此外，MIP-PVAm/CPVA微球具有良好的脱附性能，以乙醇- NaOH水溶液为洗脱液，当出水量达到20床体积（BV）时，脱附率可达99.73%。2009爱思唯尔有限公司版权所有。
Acrylamide (AM) was first graft-polymerized on the surface of crosslinked polyvinyl alcohol (CPVA) microspheres by initiating of cerium salt, and then the grafted polyacrylamide (PAM) was transformed to polyvinylamine (PVAm) via Hofmann degradation reaction, resulting in the grafted microspheres PVAm/CPVA. By adopting the novel surface molecular imprinting technique put forward by us, cholic acid molecule-imprinted material MIP-PVAm/CPVA was prepared with glutaraldehyde as crosslinking agent The binding character of MIP-PVAm/CPVA towards cholic acid molecules was studied in depth with both batch and column methods and using cholesterol as a contrast compound whose chemical structure is similar with cholic acid to a certain extent. The experimental results show that the surface-imprinted material MIP-PVAm/CPVA has excellent binding affinity and recognition selectivity for the template molecule, cholic acid. The selectivity coefficient of PVAm/CPVA microspheres (non-imprinting material) for cholic acid relative to cholesterol is only 1.314, displaying very poor recognition selectivity for cholic acid. However, after imprinting, the selectivity coefficient of MIP-PVAm/CPVA for cholic acid in respect to cholesterol is remarkably enhanced to 11.231, displaying the excellent recognition selectivity and binding affinity towards cholic acid molecules. Besides, MIP-PVAm/CPVA microspheres have fine desorption property, and by using a mixture of ethanol and NaOH aqueous solution as an eluent, the desorption ratios can reach 99.73% as the effluent amount gets up to 20 bed volumes (BV). (C) 2009 Elsevier Ltd. All rights reserved.