INDUCTION OF A MACROPHAGE-SUPPRESSIVE LYMPHOKINE BY SOLUBLE CRYPTOCOCCAL ANTIGENS AND ITS ASSOCIATION WITH MODELS OF IMMUNOLOGICAL-TOLERANCE
INDUCTION OF A MACROPHAGE-SUPPRESSIVE LYMPHOKINE BY SOLUBLE CRYPTOCOCCAL ANTIGENS AND ITS ASSOCIATION WITH MODELS OF IMMUNOLOGICAL-TOLERANCE
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DOI：
10.1128/iai.55.1.233-239.1987
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发表时间：
1987-01-01
影响因子：
3.1
通讯作者：
HALL, NK
中科院分区：
文献类型：
--
作者：
BLACKSTOCK, R;MCCORMACK, JM;HALL, NK
检测了新生隐球菌的可溶性提取物诱导巨噬细胞调节性T抑制细胞的能力，已知该细胞出现在感染隐球菌的小鼠的脾中。通过注射包膜或包膜较薄的隐球菌株的提取物来诱导抑制细胞。剂量-反应分析表明，低至25ug的可溶性胶囊多糖抗原即可诱导显著的抑制细胞活性，最大抑制作用出现在100ug剂量时。抑制细胞在注射抗原后1周内出现，并持续至少2个月。在给予耐受剂量的利凡、人丙种球蛋白和可溶性胶囊多糖抗原的动物中诱导抑制细胞。当这些相同的抗原以免疫原性形式注射时，没有检测到抑制细胞活性。因此，这种抑制机制在免疫耐受模型中是常见的，而不是隐球菌病或隐球菌壳多糖抗原所独有的。抑制细胞群产生的抑制吞噬的淋巴因子完全抑制只有一部分腹膜渗出细胞的吞噬活性。人群中的其他巨噬细胞并没有完全受到抑制，但吞噬的酵母细胞数量有所减少。
Soluble extracts of Crytococcus neoformans were examined for their ability to induce a macrophage-regulatory T-suppressor cell known to appear in the spleens of mice infected with cryptococci. Suppressor cells were induced by injection of extracts of encapsulated or thinly encapsulated strains of cryptococci. Dose-response analysis showed that as little as 25 .mu.g of soluble capsular polysaccharide antigen could induce significant suppressor cell activity, with maximum suppression occurring at a dose of 100 .mu.g. The suppressor cells appeared within 1 week of injection of antigen and persisted for at least 2 months. Suppressor cells were induced in animals given tolerogenic doses of levan, human gamma globulin, and soluble capsular polysaccharide antigen. When these same antigens were administered in immunogenic form, no suppressor cell activity was detected. Therefore, the suppressive mechanism was common to models of immunologic tolerance and was not unique to cryptococcal disease or cryptococcal capsular polysaccharide antigen. The phagocytosis-inhibiting lymphokine produced by the suppressor cell population completely inhibited the phagocytic activity of only a portion of peritoneal exudate cells. Other macrophages in the population were not totally inhibited but exhibited a reduction in the number of yeast cells engulfed.