3D printing-based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brain.
3D printing-based frugal manufacturing of glass pipettes for minimally invasive delivery of therapeutics to the brain.
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DOI：
10.1002/nep3.20
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发表时间：
2023-09
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液体形式的药剂的脑内递送通常通过市售的耐用金属针实现。然而，它们的大小和纹理可能会导致机械性脑损伤。具有细尖端的玻璃移液器可以显著减少与注射相关的脑损伤，但是需要使用昂贵的可编程移液器拉拔器。这项研究是为了消除经济障碍的应用微创输送治疗药物的大脑，如化学化合物，病毒载体和细胞。我们利用免费教育在线资源和新兴低成本3D打印机的快速发展，设计了一款价格实惠的移液器（APP），以消除成本障碍。我们表明，我们的APP可以生产具有尖锐尖端开口的玻璃移液器，其开口小于或等于20 μm，这对于将治疗剂输送到大脑中是足够薄的。本研究建立了一条从移液到脑内注射的低成本、开源设备的管道，以促进APP的应用。本着节俭科学的精神，我们的设备可以使玻璃移液大众化，并实质性地推动微创和精确控制的脑内治疗药物的应用，以寻找更有效的脑疾病治疗方法。
Intracerebral delivery of agents in liquid form is usually achieved through commercially available and durable metal needles. However, their size and texture may contribute to mechanical brain damage. Glass pipettes with a thin tip may significantly reduce injection-associated brain damage but require access to prohibitively expensive programmable pipette pullers. This study is to remove the economic barrier to the application of minimally invasive delivery of therapeutics to the brain, such as chemical compounds, viral vectors, and cells. We took advantage of the rapid development of free educational online resources and emerging low-cost 3D printers by designing an affordable pipette puller (APP) to remove the cost obstacle. We showed that our APP could produce glass pipettes with a sharp tip opening down to 20 μm or less, which is sufficiently thin for the delivery of therapeutics into the brain. A pipeline from pipette pulling to brain injection using low-cost and open-source equipment was established to facilitate the application of the APP. In the spirit of frugal science, our device may democratize glass pipette-puling and substantially promote the application of minimally invasive and precisely controlled delivery of therapeutics to the brain for finding more effective therapies of brain diseases.