Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants
Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants
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DOI：
10.1007/s004250050547
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发表时间：
1999-04-01
期刊：
影响因子：
4.3
通讯作者：
Páldi, E
中科院分区：
文献类型：
--
作者：
Janda, T;Szalai, G;Páldi, E
在正常生长条件下，向玉米幼苗 (Zea mag's L.) 的水培生长溶液中添加 0.5 mM 水杨酸 (SA)，可提供针对后续低温胁迫的保护。这一观察结果通过叶绿素荧光参数和电解质泄漏测量得到证实。此外，1天的0.5 mM SA预处理降低了生长温度（22/20摄氏度）下的净光合作用、气孔电导率和蒸腾作用。由于可变荧光与最大荧光的比率 (F-v/F-m) 仅略有下降，因此光合活性的下降并不是由于光系统 II 的抑制所致。抗氧化酶分析表明，虽然SA处理没有引起抗坏血酸过氧化物酶（EC 1.11.1.11）和超氧化物歧化酶（EC 1.15.1.1）活性的任何变化，但过氧化氢酶（EC 1.11.1.6）活性降低，愈创木酚过氧化物酶（EC 1.11.1.7）和谷胱甘肽还原酶（EC）增加。 1.6.4.2) 在 22/20 摄氏度下进行 1 天 SA 处理后的活性。在天然聚丙烯酰胺凝胶中，过氧化物酶同工酶中存在一条仅在 SA 处理的植物中才能看到的条带。这表明在正常生长温度下用SA预处理玉米植株可能会诱导抗氧化酶的产生，从而提高耐冷性。
The addition of 0.5 mM salicylic acid (SA) to the hydroponic growth solution of young maize (Zea mag's L.) plants under normal growth conditions provided protection against subsequent low-temperature stress. This observation was confirmed by chlorophyll fluorescence parameters and electrolyte leakage measurements. In addition, 1 d of 0.5 mM SA pre-treatment decreased net photosynthesis, stomatal conductivity and transpiration at the growth temperature (22/20 degrees C). Since there was only a slight decrease in the ratio of variable to maximal fluorescence (F-v/F-m) the decrease in photosynthetic activity is not due to a depression in photosystem II. The analysis of antioxidant enzymes showed that whereas SA treatment did not cause any change in ascorbate peroxidase (EC 1.11.1.11) and superoxide dismutase (EC 1.15.1.1) activities, there was a decrease in catalase (EC 1.11.1.6) activity, and an increase in guaiacol peroxidase (EC 1.11.1.7) and glutathione reductase (EC 1.6.4.2) activities after the 1-d SA treatment at 22/20 degrees C. In native polyacrylamide gels there was, among the peroxidase isoenzymes, a band which could be seen only in SA-treated plants. It is suggested that the pre-treatment of maize plants with SA at normal growth temperature may induce antioxidant enzymes which lead to increased chilling tolerance.