Identification of circadian gene expression in the rat pineal gland and retina by mRNA differential display.
Identification of circadian gene expression in the rat pineal gland and retina by mRNA differential display.
复制标题
通过 mRNA 差异显示鉴定大鼠松果体和视网膜中昼夜节律基因的表达。
DOI：
10.1016/0304-3940(95)11331-p
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发表时间：
1995
影响因子：
2.5
通讯作者：
Craft,CM
中科院分区：
文献类型：
--
作者：
Gauer,F;Kedzierski,W;Craft,CM
为了识别对昼夜节律和明暗调节至关重要的独特基因产物，我们使用差异显示技术研究了从主观白天（D）或主观夜间（N）收集的大鼠松果体和视网膜中分离出的mRNA。使用设计的引物和逆转录酶-聚合酶链式反应从松果体扩增出约 50 个 mRNA 的亚群。 35 个 mRNA 在 D 和 N 样品中表达相同。根据主观夜间的较高表达（N310、N320、N383、N420），从显示差异表达的 15 个 mRNA 中选择了四个扩增产物，并对每个产物进行亚克隆和测序。在所研究的四种 cDNA 中，只有 N310 被确定与已知的细胞粘附蛋白（称为 F3）具有显着的同一性。该蛋白在发育中的小鼠神经元中高度表达，并且与鸡接触素相关。正在进一步检查所识别的每种差异显示产品，以了解它们在松果体和视网膜活动昼夜节律调节中的潜在意义。
To identify unique gene products that could be crucial for circadian and light-dark regulations, using the technology of differential display, we have investigated mRNAs that were isolated from rat pineals and retinas collected either during subjective day (D) or subjective night (N). A subpopulation of about 50 mRNAs were amplified from pineal using designed primers and reverse transcriptase-polymerase chain reaction. Thirty five of the mRNAs were expressed equivalently in both D and N samples. From the 15 mRNAs showing differential expression, four amplified products were selected, based on higher expression during the subjective night (N310, N320, N383, N420) and each was subcloned and sequenced. Of the four cDNAs studied, only N310 was determined to have significant identity to a known cell adhesion protein, called F3. This protein is highly expressed in developing mouse neurons and is related to chicken contactin. Each differentially displayed product identified is being examined further in order to understand their potential significance in the circadian regulation of pineal and retinal activities.