Mechanisms of activation and suppression in rat Nb 2 lymphoma cells: a model for interactions between prolactin and the immune system.
Mechanisms of activation and suppression in rat Nb 2 lymphoma cells: a model for interactions between prolactin and the immune system.
复制标题
大鼠 Nb 2 淋巴瘤细胞的激活和抑制机制：催乳素与免疫系统之间相互作用的模型。
DOI：
10.1006/excr.1995.1192
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Mastro,AM
中科院分区：
文献类型：
--
作者：
Bates,LG;Grove,DS;Mastro,AM
大鼠Nb 2淋巴瘤细胞已被广泛用于人生长激素和多种催乳素的生物测定。因为它们的形态学特征表明T细胞谱系，Nb 2细胞进行了检查，他们对T细胞有丝分裂原伴刀豆球蛋白A，美洲商陆有丝分裂原，和植物血凝素P的反应。正如预期的那样，大鼠催乳素的剂量反应进行了观察，但是，试图诱导增殖使用常规的T细胞有丝分裂原在浓度正常刺激大鼠初级淋巴细胞失败。此外，当Nb 2细胞同时与凝集素和次优浓度的催乳素一起孵育时，用植物血凝素P和美洲商陆有丝分裂原观察到催乳素的刺激作用的剂量依赖性抑制，但用伴刀豆球蛋白A则没有。催乳素刺激的Nb 2细胞的培养液中也含有一种抑制正常大鼠淋巴细胞被刀豆球蛋白A激活的因子。该因子不阻断IL-2受体的诱导，并且IL-2依赖性CTLL-2细胞的增殖可以通过外源性IL-2恢复。由于Nb 2细胞从催乳素依赖性淋巴结肿瘤演变而来，这些结果可能对进一步了解垂体激素，特别是催乳素在对催乳素依赖性肿瘤进展的免疫反应中的作用有影响。
Rat Nb 2 lymphoma cells have been widely used to bioassay human growth hormone and many species of prolactin. Because their morphologic characterization suggests a T-cell lineage, Nb 2 cells were examined for their response to the T-cell mitogens concanavalin A, pokeweed mitogen, and phytohemagglutinin P. As expected, a dose-response to rat prolactin was observed; however, attempts to induce proliferation using the conventional T-cell mitogens failed at concentrations normally stimulatory for rat primary lymphocytes. Moreover, when Nb 2 cells were simultaneously incubated with lectin plus a suboptimal concentration of prolactin, a dose-dependent suppression of the stimulatory effects of prolactin was observed with phytohemagglutinin P and pokeweed mitogen, although not with concanavalin A. Culture medium of prolactin-stimulated Nb 2 cells also contained a factor which inhibited normal rat lymphocyte activation by concanavalin A. The factor did not block induction of the IL-2 receptor and proliferation of IL-2-dependent CTLL-2 cells could be restored by exogenous IL-2. Because Nb 2 cells evolved from a lactogen-dependent lymph node tumor, these results may have implications for further understanding the role of pituitary hormones, particularly prolactin, in the immune response to hormone-dependent tumor progression.