Effective charge of bovine serum albumin determined by electrophoresis NMR
Effective charge of bovine serum albumin determined by electrophoresis NMR
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DOI：
10.1016/j.cplett.2006.12.068
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发表时间：
2007-02-19
影响因子：
2.8
通讯作者：
Scheler, Ulrich
中科院分区：
文献类型：
--
作者：
Boehme, Ute;Scheler, Ulrich
本文用动态核磁共振法测定了牛血清白蛋白在水溶液中的有效电荷随pH的变化，有效电荷是由解离度随pH的变化和局部反离子缩合作用引起的。而流体动力学摩擦是由脉冲场梯度NMR测量的扩散系数确定的，电泳迁移率是由电泳NMR确定的。每个分子的有效电荷是从流体动力学摩擦力和电场中作用在电荷上的力之间的力平衡计算的。由于它受反离子缩合的影响，因此它小于滴定的净电荷。(c)2006 Elsevier B.V.保留所有权利。
The effective charge of bovine serum albumin in aqueous solution is determined by dynamic NMR as a function of pH. The effective charge results from the degree of dissociation varying with pH and the local counterion condensation. While the hydrodynamic friction is determined from the diffusion coefficient measured by pulsed-field gradient NMR, the electrophoretic mobility is determined by electrophoresis NMR. The effective charge per molecule is calculated from the force balance between hydrodynamic friction and force in the electric field acting on the charges. Since it is influenced by counterion condensation it is smaller than the net charge from titration. (c) 2006 Elsevier B.V. All rights reserved.