Assessing the impact of the biological control agent Bacillus thuringiensis on the indigenous microbial community within the pepper plant phyllosphere.
Assessing the impact of the biological control agent Bacillus thuringiensis on the indigenous microbial community within the pepper plant phyllosphere.
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DOI：
10.1111/j.1574-6968.2008.01178.x
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发表时间：
2008-07
影响因子：
2.1
通讯作者：
Baoguo Zhang;Z. Bai;D. Hoefel;L. Tang;Zhiguang Yang;G. Zhuang;Jianzhou Yang;Hongxun Zhang
中科院分区：
文献类型：
--
作者：
Baoguo Zhang;Z. Bai;D. Hoefel;L. Tang;Zhiguang Yang;G. Zhuang;Jianzhou Yang;Hongxun Zhang
尽管生物防治剂（BCAs）已被广泛用于控制植物的昆虫和病原体，但关于此类药剂对植物叶圈内的土著微生物群落的影响知之甚少。我们评估了BCA苏云金芽孢杆菌（Bt）对辣椒植物叶际内的微生物群落的影响，使用文化独立的方法。磷脂脂肪酸（PLFA）分析表明，细菌和真菌的生物量没有显着影响后Bt的应用。然而，PLFA数据的主成分分析表明，Bt确实改变了叶际微生物群落结构显着。16 S rRNA基因导向的PCR结合变性梯度凝胶电泳（DGGE）也表明，接种Bt后叶际细菌群落结构发生了显著变化。切下的DGGE带的系统发育分析表明细菌门的变化，从未处理的样品带主要属于厚壁菌门，而γ-变形菌丰富的处理过的样品。
Although biological control agents (BCAs) have been used extensively for controlling insects and pathogens of plants, little is known regarding the effects of such agents on the indigenous microbial communities within the plant phyllosphere. We assessed the effect of the BCA Bacillus thuringiensis (Bt) on the microbial communities within the pepper plant phyllosphere using culture-independent methodologies. Phospholipid fatty acid (PLFA) analysis suggested that the bacterial and fungal biomass were not significantly affected following Bt application. However, principal component analysis of PLFA data indicated that Bt did change the phyllosphere microbial community structure significantly. 16S rRNA gene-directed PCR with denaturing gradient gel electrophoresis (DGGE) also suggested a significant change in the phyllosphere bacterial community structure following Bt inoculation. Phylogenetic analysis of excised DGGE bands suggested a change in bacterial phyla; bands from untreated samples predominantly belonged to the Firmicutes, while Gammaproteobacteria abounded in the treated samples.