Multiplicity of functions for the otu gene products during Drosophila oogenesis.
Multiplicity of functions for the otu gene products during Drosophila oogenesis.
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DOI：
10.1002/dvg.1020090203
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
P. Storto;R. King
中科院分区：
文献类型：
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作者：
P. Storto;R. King
卵巢肿瘤基因的行为就好像它编码一种产物（OGP），这是卵原细胞转化为功能性卵母细胞的几个早期步骤所必需的。已经研究了17种甲基磺酸乙酯诱导的突变，它们的突变表型可以解释为个体生殖细胞对突变生殖细胞自身合成的不同水平的OGP的分级反应。最低和最高水平的OGP似乎分别由otu 10和otu 14产生。15个OGP水平中等的突变体对温度敏感;低于正常的温度促进卵巢发育，而高于正常的温度抑制卵巢发育。这些突变体的一个亚组在10 B期期间不能在其养育细胞的皮质细胞质中形成肌动蛋白微丝束系统，并且这些缺陷的养育细胞不能将其细胞质运输到卵母细胞，如通常在阶段10 B和12之间发生的。除了在肌动蛋白介导的营养细胞胞质运输中的作用外，OGP似乎还改变了巨大多线染色体的形态，这些染色体是在营养细胞进行DNA复制的内循环时形成的。遗传证据表明，otu还编码第二种产物（SP），在卵子发生后期被利用。SP是在卵质中合成富含糖原的β卵黄球所必需的。otu基因的产物在胚胎发生中也起着重要但未知的作用。
The ovarian tumor gene behaves as if it encodes a product (OGP), which is required during several early steps in the transformation of oogonia into functional oocytes. Seventeen ethyl methane sulfonate-induced mutations have been studied, and their mutant phenotypes can be explained as graded responses by individual germ cells to different levels of OGP synthesized by the mutant germ cells themselves. The lowest and highest levels of OGP appear to be produced by otu10 and otu14, respectively. The 15 mutants with intermediate OGP levels are temperature sensitive; subnormal temperatures improve ovarian development, while above-normal temperatures suppress it. A subgroup of these mutants are unable to form a system of actin microfilament bundles in the cortical cytoplasm of their nurse cells during stage 10B, and these defective nurse cells are unable to transport their cytoplasm to the oocyte, as normally happens between stages 10B and 12. In addition to its role in the actin-mediated transport of nurse cell cytoplasm, OGP also appears to alter the morphology of giant polytene chromosomes, which form as the nurse cells undergo endocycles of DNA replication. Genetic evidence suggests that otu also encodes a second product (SP) that is utilized late in oogenesis. SP is required for the synthesis in the ooplasm of glycogen-rich, beta yolk spheres. Products of the otu gene also play a vital but unknown role in embryogenesis.