Comparison of the effects of metals on cellular injury and lipid peroxidation in isolated rat hepatocytes.
Comparison of the effects of metals on cellular injury and lipid peroxidation in isolated rat hepatocytes.
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比较金属对离体大鼠肝细胞细胞损伤和脂质过氧化的影响。
DOI：
10.1080/15287398109529965
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Klaassen,CD
中科院分区：
文献类型：
--
作者：
Stacey,NH;Klaassen,CD
各种机制，包括脂质过氧化的增加，已经被提出来解释金属诱导的细胞损伤。通过比较同一细胞群中的几种金属，可以确定产生细胞损伤的能力与改变特定机制参数的能力之间是否存在相关性。本研究特别感兴趣的是金属诱导的细胞毒性与脂质过氧化增加之间的关系。因此，通过评估细胞内K+和谷草转氨酶（AST）的损失，研究了终浓度为1-1000 μM的Cr、Mn、Zn、Ni、Pb、Se、V、Fe、Cd、Hg和Cu对分离肝细胞活力的影响。同时，通过硫代巴比妥酸（TBA）反应物的增加，对金属诱导脂质过氧化的能力进行了评估。汞和铜对细胞损伤的影响最低，而镉对细胞活力的影响较小，而铁在1000 μM时对细胞内K+的影响较小。在存在V时，脂质过氧化的绝对增幅最大，其次是Fe和Hg， Cd和Se引起TBA反应物的增幅最小。这些观察结果表明，与镉和汞相关的脂质过氧化不一定是导致这两种金属诱导的细胞活力丧失的原因。
Various mechanisms, including increases in lipid peroxidation, have been proposed to account for metal‐induced cellular injury. By comparing several metals in the same cell population, it is possible to determine whether a correlation exists between ability to produce cell injury and ability to alter parameters pertaining to a particular mechanism. Of particular interest in this study was the relation between metal‐induced cytotoxicity and increases in lipid peroxidation. The effects of Cr, Mn, Zn, Ni, Pb, Se, V, Fe, Cd, Hg, and Cu, at final concentrations of 1–1000 μM, on the viability of isolated hepatocytes were therefore examined by assessing the loss of intraceliuiar K+and aspartate aminotransferase (AST). Simultaneously, the ability of the metals to induce lipid peroxidation, as measured by an increase in thiobarbituric acid (TBA) reactants, was assessed. Hg and Cu required the lowest concentration to produce cellular injury, while Cd produced less dramatic changes in cell viability and Fe at 1000 μM produced only a small decrease in intraceliuiar K+. The largest absolute increases in lipid peroxidation were found in the presence of V, followed by Fe and Hg, with Cd and Se causing the smallest increase in TBA reactants. These observations suggest that the lipid peroxidation associated with Cd and Hg is not necessarily responsible for the loss of cell viability induced by these two metals.