Star Polymers with Designed Reactive Oxygen Species Scavenging and Agent Delivery Functionality Promote Plant Stress Tolerance
Star Polymers with Designed Reactive Oxygen Species Scavenging and Agent Delivery Functionality Promote Plant Stress Tolerance
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DOI：
10.1021/acsnano.1c10828
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发表时间：
2022-03-22
期刊：
影响因子：
17.1
通讯作者：
Lowry, Gregory, V
中科院分区：
文献类型：
--
作者：
Zhang, Yilin;Fu, Liye;Lowry, Gregory, V
植物非生物胁迫诱导活性氧（ROS）在叶片中积累，降低光合性能和作物产量。清除活性氧并同时在体内提供营养的物质需要控制这种应激。在这里，我们将清除ROS和释放ROS触发剂的功能结合到一个类似于20 nm的ROS响应星形聚合物（RSP）聚（丙烯酸）-嵌段聚（（2-（甲基亚砜基）丙烯酸乙酯）-co-（2-（甲基硫代）丙烯酸乙酯））（PAA-b-P(MSEA-co-MTEA)）中，通过同时清除ROS和释放营养剂来缓解植物胁迫。高光谱成像结果表明，所有的RSP都能穿透番茄叶片表皮，其中32.7%的RSP与叶肉叶绿体结合。RSP在体外清除10 μ mol mg（-1）的活性氧，在体内抑制活性氧。RSP与H2O2的体外反应促进了星形聚合物中营养剂（Mg2+）的释放。与未处理对照相比，叶面施用RSP增加了热光胁迫下植物的光合作用，提高了碳同化、CO2同化量子产率、Rubisco羧化速率和光系统II量子产率。负载Mg的RSP改善了缺镁植株的光合作用，主要是通过促进Rubisco活性。这些结果表明，像RSP这样的活性氧清除纳米载体有可能缓解作物植物的非生物胁迫，使作物植物对热胁迫和其他气候变化引起的非生物胁迫具有更强的适应性。
Plant abiotic stress induces reactive oxygen species (ROS) accumulation in leaves that can decrease photosynthetic performance and crop yield. Materials that scavenge ROS and simultaneously provide nutrients in vivo are needed to manage this stress. Here, we incorporated both ROS scavenging and ROS triggered agent release functionality into an similar to 20 nm ROS responsive star polymer (RSP) poly(acrylic acid)-block-poly((2-(methylsulfinyl)ethyl acrylate)-co-(2-(methylthio)ethyl acrylate)) (PAA-b-P(MSEA-co-MTEA)) that alleviated plant stress by simultaneous ROS scavenging and nutrient agent release. Hyper- spectral imaging indicates that all of the RSP penetrates through the tomato leaf epidermis, and 32.7% of the applied RSP associates with chloroplasts in mesophyll. RSP scavenged up to 10 mu mol mg(-1) ROS in vitro and suppressed ROS in vivo in stressed tomato (Solanum lycopersicum) leaves. Reaction of the RSP with H2O2 in vitro enhanced the release of nutrient agent (Mg2+) from star polymers. Foliar applied RSP increased photosynthesis in plants under heat and light stress compared to untreated controls, enhancing the carbon assimilation, quantum yield of CO2 assimilation, Rubisco carboxylation rate, and photosystem II quantum yield. Mg loaded RSP improved photosynthesis in Mg deficient plants, mainly by promoting Rubisco activity. These results indicate the potential of ROS scavenging nanocarriers like RSP to alleviate abiotic stress in crop plants, allowing crop plants to be more resilient to heat stress, and potentially other climate change induced abiotic stressors.